The transient nature of humic substances: changes in their molecular composition during stimulated microbial composting and Agaricus bisporus growth

IF 4.3 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Biogeochemistry Pub Date : 2026-02-20 DOI:10.1007/s10533-026-01309-z

Katharina Duran, Nick Quist, Johan J. P. Baars, Thomas W. Kuyper, Mirjam A. Kabel, Rob N. J. Comans

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引用次数: 0

Abstract

The substrate production process for the edible mushroom Agaricus bisporus offers a controlled model environment to investigate organic matter transformation. Despite the central role of humic substances (HS) in organic matter dynamics, detailed insights into their compositional changes during decomposition are limited. We investigated the formation and transformation of dissolved and solid phase HS during microbial composting and subsequent mycelial growth of A. bisporus. Total carbon and nitrogen were analyzed in both bulk material and HS fractions. Additionally, bulk material was analyzed for carbohydrates, fatty acids, and lignin content and composition. Pyrolysis–GC–MS was used to characterize humic acids (HA), fulvic acids (FA), and the bulk material. Throughout microbial composting HS formed, with HA as main fraction followed by FA. The formation of HS coincided with substantial degradation of cellulosic components and enrichment of organic nitrogen. HA was particularly enriched in lignin(derived) and nitrogen-containing compounds. During early mycelial growth of A. bisporus HS and particularly HA decreased, thus HS are an accessible source of carbon and nitrogen for fungal metabolism.

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腐殖质的瞬时性质：在受刺激的微生物堆肥和双孢蘑菇生长过程中其分子组成的变化

食用菌双孢蘑菇的基质生产过程为研究有机物质转化提供了可控的模型环境。尽管腐殖质（HS）在有机物动力学中的核心作用，但在分解过程中对其组成变化的详细见解是有限的。研究了双孢酵母在微生物堆肥和菌丝生长过程中溶相和固相HS的形成和转化。对散料和HS馏分的总碳、总氮进行了分析。此外，还分析了散装物料的碳水化合物、脂肪酸和木质素的含量和组成。采用热裂解-气相色谱-质谱法对腐植酸（HA）、黄腐酸（FA）及物料进行表征。在整个微生物堆肥过程中形成HS， HA为主要组分，FA次之。HS的形成与纤维素组分的大量降解和有机氮的富集同时发生。HA尤其富含木质素（衍生）和含氮化合物。在双孢霉菌丝生长早期，HS尤其是HA含量下降，因此HS是真菌代谢碳氮的可及来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biogeochemistry 环境科学-地球科学综合

CiteScore

7.10

自引率

5.00%

发文量

112

审稿时长

3.2 months

期刊介绍： Biogeochemistry publishes original and synthetic papers dealing with biotic controls on the chemistry of the environment, or with the geochemical control of the structure and function of ecosystems. Cycles are considered, either of individual elements or of specific classes of natural or anthropogenic compounds in ecosystems. Particular emphasis is given to coupled interactions of element cycles. The journal spans from the molecular to global scales to elucidate the mechanisms driving patterns in biogeochemical cycles through space and time. Studies on both natural and artificial ecosystems are published when they contribute to a general understanding of biogeochemistry.