c-Myc regulated long noncoding RNA TRD-AS1 to affect the progression of oral squamous cell carcinoma.

IF 1.7 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Genes & genomics Pub Date : 2026-05-01 Epub Date: 2026-03-10 DOI:10.1007/s13258-026-01745-z

Ziyue Feng, Yuxuan Han, Xiaofeng Qin, Miaoting Fu, Xiangyang Li, Youming Zhu

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引用次数: 0

Abstract

Background: Oral squamous cell carcinoma (OSCC) progression is closely associated with dysregulated oncogenic transcription programs and noncoding RNA networks. c-Myc is a key transcription factor in tumor biology, but the role of c-Myc-regulated long noncoding RNAs such as TRD-AS1 (LNC TRD-AS1) in OSCC progression remains to be clarified.

Objective: To investigate the effect of c-Myc-regulated LNC TRD-AS1 on the progression of OSCC.

Methods: The effects of c-Myc on LNC TRD-AS1 were detected. Expression of c-Myc and LNC TRD-AS1 in OSCC and paracancerous tissues. Dual luciferase reporter assay verified the binding of c-Myc to the promoter region of LNC TRD-AS1. The localization of LNC TRD-AS1 in OSCC was determined by nucleocytoplasmic isolation assay and RNA FISH experiments. The effects of overexpression, knockdown of LNC TRD-AS1 and rescue experiments on migration, metabolism, and proliferation of OSCC cells were observed by quantitative real-time polymerase chain reaction (qRT-PCR), western blots, scratch tests, Transwell assays, medium color and pH changes, ATP measurement, CCK-8 assays, and colony formation assays.

Results: It was found that c-Myc positively regulated the expression of LNC TRD-AS1 in OSCC cell lines and tissues. The binding of c-Myc to the LNC TRD-AS1 promoter region was verified by dual-luciferase reporter assays. Both nucleoplasmic separation assays and RNA FISH showed that LNC TRD-AS1 was localized to the nuclei in OSCC cells. Overexpression of LNC TRD-AS1 promoted migration, metabolism and proliferation in OSCC cell lines, while knockdown had the opposite effect.

Conclusion: c-Myc positively regulated LNC TRD-AS1 in OSCC. LNC TRD-AS1 affected the migration, proliferation and metabolism of OSCC by affecting the tumor acid metabolism.

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c-Myc调节长链非编码RNA TRD-AS1影响口腔鳞状细胞癌的进展。

背景：口腔鳞状细胞癌（OSCC）的进展与癌基因转录程序和非编码RNA网络的失调密切相关。c-Myc是肿瘤生物学中的关键转录因子，但c-Myc调控的长链非编码rna如TRD-AS1 （LNC TRD-AS1）在OSCC进展中的作用尚不清楚。目的：探讨c- myc调控的LNC TRD-AS1对OSCC进展的影响。方法：检测c-Myc对LNC TRD-AS1的影响。c-Myc和LNC TRD-AS1在鳞癌及癌旁组织中的表达。双荧光素酶报告试验证实了c-Myc与LNC TRD-AS1启动子区域的结合。通过核细胞质分离实验和RNA FISH实验确定LNC TRD-AS1在OSCC中的定位。通过实时荧光定量聚合酶链式反应（qRT-PCR）、western blots、划痕试验、Transwell试验、培养基颜色及pH变化、ATP测定、CCK-8测定、菌落形成试验，观察LNC TRD-AS1过表达、敲低及挽救实验对OSCC细胞迁移、代谢及增殖的影响。结果：发现c-Myc在OSCC细胞系和组织中正调控LNC TRD-AS1的表达。双荧光素酶报告基因检测证实了c-Myc与LNC TRD-AS1启动子区域的结合。核质分离实验和RNA FISH均表明，LNC TRD-AS1在OSCC细胞中定位于细胞核。LNC TRD-AS1过表达促进了OSCC细胞系的迁移、代谢和增殖，而敲低则相反。结论：c-Myc在OSCC中正调控LNC TRD-AS1。LNC TRD-AS1通过影响肿瘤酸代谢影响OSCC的迁移、增殖和代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genes & genomics 生物-生化与分子生物学

CiteScore

3.70

自引率

4.80%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Genes & Genomics is an official journal of the Korean Genetics Society (http://kgenetics.or.kr/). Although it is an official publication of the Genetics Society of Korea, membership of the Society is not required for contributors. It is a peer-reviewed international journal publishing print (ISSN 1976-9571) and online version (E-ISSN 2092-9293). It covers all disciplines of genetics and genomics from prokaryotes to eukaryotes from fundamental heredity to molecular aspects. The articles can be reviews, research articles, and short communications.