Stability of Bence Jones protein in refrigerated urine samples over five days: a preanalytical evaluation.

IF 1.9 Q2 MEDICINE, GENERAL & INTERNAL

Diagnosis Pub Date : 2026-03-09 eCollection Date: 2026-08-01 DOI:10.1515/dx-2025-0183

Maria Rita Cozzi, Fabio Del Ben, Martina Amato, Romina Boschian, Claudio Buciol, Chiara Corso, Cinzia Crepaldi, Tamara Damian, Riccardo De Appollonia, Katya Gaspardo, Fabiana Giordari, Matteo Turetta, Francesco Volpi, Agostino Steffan

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引用次数: 0

Abstract

Objectives: This study investigates the short-term stability of Bence Jones protein (BJP) in refrigerated urine samples stored over a period of 5 days. The aim was to evaluate whether BJP concentrations remain consistent when urine specimens are refrigerated and analyzed over time.

Methods: Urine samples were collected from 20 plasma cell dyscrasia patients with BJP for a total of 30 independent time-series and 83 data-points. Measurements were performed at five time points: Day 0 (baseline), and Days 1 through 4. Percentage difference (PD%) from baseline was used to quantify temporal change.

Results: The median baseline BJP M-spike percentage was 54 % (range: 17-87 %), covering a wide range of total urinary protein (median 296 mg/L, range 64-3,094 mg/L). PD% ranged from -2 % on Day 3 to +7 % on Day 1, with no statistically significant trend over time. The derived instability equation, PD%=0.33 × time (days), yielded r= -0.03 and p=0.77, indicated negligible instability.

Conclusions: These findings demonstrate acceptable short-term stability of BJP in refrigerated urine over 5 days, supporting its use in routine laboratory workflows involving delayed analysis.

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5天内冷冻尿液样本中Bence Jones蛋白的稳定性：分析前评估。

目的：研究本·琼斯蛋白（Bence Jones protein， BJP）在冷藏尿液样本中保存5天的短期稳定性。目的是评估当尿液标本冷藏和分析一段时间后，BJP浓度是否保持一致。方法：收集20例BJP浆细胞病变患者尿液样本，共30个独立时间序列，83个数据点。测量在五个时间点进行：第0天（基线）和第1至第4天。与基线的百分比差异（PD%）用于量化时间变化。结果：基线BJP M-spike百分比中位数为54 %（范围：17-87 %），涵盖了广泛的尿蛋白范围（中位数296 mg/L，范围64-3,094 mg/L）。PD%从第3天的-2 %到第1天的+7 %不等，随着时间的推移没有统计学上的显著趋势。导出的不稳定性方程PD%=0.33 ×时间（天），r= -0.03, p=0.77，表明不稳定性可以忽略不计。结论：这些发现表明，人民党在冷藏尿液中5天以上的短期稳定性是可以接受的，支持其在常规实验室工作流程中使用，包括延迟分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnosis MEDICINE, GENERAL & INTERNAL-

CiteScore

7.20

自引率

5.70%

发文量

期刊介绍： Diagnosis focuses on how diagnosis can be advanced, how it is taught, and how and why it can fail, leading to diagnostic errors. The journal welcomes both fundamental and applied works, improvement initiatives, opinions, and debates to encourage new thinking on improving this critical aspect of healthcare quality.　 Topics: -Factors that promote diagnostic quality and safety -Clinical reasoning -Diagnostic errors in medicine -The factors that contribute to diagnostic error: human factors, cognitive issues, and system-related breakdowns -Improving the value of diagnosis – eliminating waste and unnecessary testing -How culture and removing blame promote awareness of diagnostic errors -Training and education related to clinical reasoning and diagnostic skills -Advances in laboratory testing and imaging that improve diagnostic capability -Local, national and international initiatives to reduce diagnostic error