HAUS1-mediated activation of CDK4 transcription enhances proliferation, invasion and migration in hepatocellular carcinoma.

Abstract

Hepatocellular carcinoma (HCC) is distinguished by aggressive proliferation, invasion, and migration, with its underlying molecular mechanisms remaining largely elusive.This study delves into the molecular mechanisms underlying HAUS1's role in HCC progression, focusing on its regulatory relationship with Cyclin-Dependent Kinase 4 (CDK4). We analyzed 34 HCC tissue specimens and conducted in vitro assays to evaluate HAUS1's impact on cell proliferation, invasion, migration, cycle and apoptosis. Potential molecules regulated by HAUS1 that could influence HCC function were further screened. Notably, HAUS1 was shown to activate CDK4 (Cyclin-Dependent Kinase 4) transcription, establishing a regulatory nexus where HAUS1-induced CDK4 expression exacerbates HCC malignancy. In vivo studies reinforced these findings, evidencing that HAUS1 influences tumor growth in xenograft models. Collectively, our research elucidates HAUS1's oncogenic role in HCC, mediated through CDK4 activation, and highlights its potential as a therapeutic target in HCC therapy. Mechanistic diagram illustrating how HAUS1 activates CDK4 transcription to promote proliferation, invasion and migration in HCC.