Chitosan and Gold Nanoparticle-Based Coatings for Electrophoretic Separation of Biologically Active Compounds

IF 1.6 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2025-12-08 DOI:10.1007/s10337-025-04462-z

E. R. Ziangirova, E. A. Kolobova, E. V. Solovyeva, L. A. Kartsova

引用次数: 0

Abstract

Two types of physically adsorbed hybrid coatings for the inner walls of fused silica capillaries using chitosan and gold nanoparticles (GNPs) are proposed for the selective electrophoretic separation of biologically active substances. The first approach involves the formation of a coating with chitosan-modified citrate-stabilized GNPs (cGNPs@CS). The second approach involves the sequential deposition of positively charged polydiallyldimethylammonium chloride (PDDA) and negatively charged citrate-stabilized gold nanoparticles (cGNPs) followed by functionalization with chitosan. The resulting cGNPs@CS-based coatings effectively shield capillary silanol groups in the pH range of 2 to 7, which is unattainable with a chitosan monolayer. The hybrid coatings provided high resolution of dansylated amino acid derivatives and increased resolution factors in the electrophoretic separation of carvedilol enantiomers.

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壳聚糖和金纳米颗粒涂层用于生物活性化合物的电泳分离

提出了壳聚糖和纳米金在熔融二氧化硅毛细管内壁的两种物理吸附杂化涂层，用于生物活性物质的选择性电泳分离。第一种方法是用壳聚糖修饰的柠檬酸稳定GNPs形成涂层（cGNPs@CS）。第二种方法是将带正电的聚二烯基二甲基氯化铵（PDDA）和带负电的柠檬酸盐稳定的金纳米粒子（cGNPs）依次沉积，然后用壳聚糖进行功能化。所得cGNPs@CS-based涂层在pH为2 ~ 7的范围内有效地屏蔽了毛细管硅醇基团，这是壳聚糖单层膜无法实现的。杂化涂层提供了高分辨率的丹酰化氨基酸衍生物，并增加了卡维地洛对映体电泳分离的分辨率因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.