Preparation and Characterization of Quaternized Polystyrene-Divinylbenzene Stationary Phases in Deep Eutectic Solvents for Anion-Exchange Chromatography

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2025-12-20 DOI:10.1007/s10337-025-04471-y

Zhongping Huang, Jianfeng Zou, Junhao Zhang, Haodong Wang, Huijun Liu

{"title":"Preparation and Characterization of Quaternized Polystyrene-Divinylbenzene Stationary Phases in Deep Eutectic Solvents for Anion-Exchange Chromatography","authors":"Zhongping Huang, Jianfeng Zou, Junhao Zhang, Haodong Wang, Huijun Liu","doi":"10.1007/s10337-025-04471-y","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, quaternized cationic polyelectrolytes were successfully decorated on polystyrene-divinylbenzene (PS-DVB) microspheres in deep eutectic solvents (DESs). Quaternized cationic polyelectrolytes and hyperbranched layers were prepared using 1,4-butanediol diglycidyl ether (BDDE) and methylamine (MA) as monomers. The effects of different reaction solvents, the amount of agglomerated polyelectrolytes, and the number of hyperbranched layers on the chromatographic performance of the stationary phase were investigated. The surface morphological feature and chemical composition of the PS-DVB microspheres were characterized by SEM, FT-IR, and elemental analysis. The results showed that the optimized anion exchanger had a good chromatographic performance by choosing a mixture of 2.0 mmol/L sodium carbonate and 2.0 mmol/L sodium bicarbonate as the mobile phase at a flow rate of 1.0 mL/min. Seven common anions (fluoride ion, chloride ion, nitrite ion, bromide ion, nitrate ion, phosphate ion, and sulfate ion) were well separated within 25 min. The average numbers of theoretical plates per meter for three batches of columns prepared in DESs increased by 5% ~ 20%, as compared with those of the columns prepared in deionized water. Additionally, the asymmetry factors of detected anions were also improved for the columns prepared in DESs.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"89 3","pages":"239 - 245"},"PeriodicalIF":1.3000,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromatographia","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s10337-025-04471-y","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, quaternized cationic polyelectrolytes were successfully decorated on polystyrene-divinylbenzene (PS-DVB) microspheres in deep eutectic solvents (DESs). Quaternized cationic polyelectrolytes and hyperbranched layers were prepared using 1,4-butanediol diglycidyl ether (BDDE) and methylamine (MA) as monomers. The effects of different reaction solvents, the amount of agglomerated polyelectrolytes, and the number of hyperbranched layers on the chromatographic performance of the stationary phase were investigated. The surface morphological feature and chemical composition of the PS-DVB microspheres were characterized by SEM, FT-IR, and elemental analysis. The results showed that the optimized anion exchanger had a good chromatographic performance by choosing a mixture of 2.0 mmol/L sodium carbonate and 2.0 mmol/L sodium bicarbonate as the mobile phase at a flow rate of 1.0 mL/min. Seven common anions (fluoride ion, chloride ion, nitrite ion, bromide ion, nitrate ion, phosphate ion, and sulfate ion) were well separated within 25 min. The average numbers of theoretical plates per meter for three batches of columns prepared in DESs increased by 5% ~ 20%, as compared with those of the columns prepared in deionized water. Additionally, the asymmetry factors of detected anions were also improved for the columns prepared in DESs.

Abstract Image

查看原文本刊更多论文

深层共晶溶剂中季铵化聚苯乙烯-二乙烯基苯固定相的制备及表征

在深共晶溶剂（DESs）中，成功地将季铵盐化的阳离子聚电解质修饰在聚苯二乙烯基苯（PS-DVB）微球上。以1,4-丁二醇二缩水甘油醚（BDDE）和甲胺（MA）为单体，制备了季铵化阳离子聚电解质和超支化层。考察了不同反应溶剂、聚电解质团聚量和超支化层数对固定相色谱性能的影响。采用SEM、FT-IR和元素分析对PS-DVB微球的表面形貌和化学成分进行了表征。结果表明，选择2.0 mmol/L碳酸钠和2.0 mmol/L碳酸氢钠的混合物为流动相，流速为1.0 mL/min时，优化后的阴离子交换剂具有良好的色谱性能。7种常见阴离子（氟离子、氯离子、亚硝酸盐离子、溴化物离子、硝酸盐离子、磷酸盐离子、硫酸盐离子）在25分钟内被很好地分离。与去离子水制备的色谱柱相比，DESs制备的三批色谱柱平均每米理论板数提高了5% ~ 20%。此外，在DESs制备的色谱柱中，检测到的阴离子的不对称因子也得到了改善。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.

文献相关原料

原料信息

原料厂家

查看厂家1