Fabrication of chitosan/polyvinyl alcohol nanofibrous membrane incorporated with Garcinia mangostana L. peel extract against Staphylococcus aureus and Pseudomonas aeruginosa isolated from canine wounds

Abstract

The electrospinning method was used to fabricate a nanofibrous membrane from chitosan/polyvinyl alcohol (CS/PVA). Afterward, Garcinia mangostana L. peel extract (MG) at a concentration of 150 mg/mL was added to the CS/PVA membrane by using a coating technique to obtain CS/PVA-MG membrane. The diameter of the nanofibers ranged from 200 to 390 nm. The antibacterial activity of the resulting CS/PVA-MG nanofibrous membrane was evaluated using agar diffusion and semi-solid media. The inhibition zone varied from 16.67 to 18.00 mm on S. aureus and 11.00 to 12.67 mm against P. aeruginosa. In a semi-solid medium, the CS/PVA-MG nanofibrous membrane completely inhibited the growth of both bacterial strains. The results demonstrate the effective inhibition of S. aureus and P. aeruginosa by CS/PVA nanofibrous membrane incorporated with 150 mg/mL of Garcinia mangostana L. peel extract, suggesting their potential application in the treatment of infected wounds on animals.