Effects of Non-Ionic Surfactant Tween 80 on Enzymatic Saccharification of Avicel and Steam-Exploded Poplar at High Solid Loading

IF 3.4 4区工程技术 Q2 ENGINEERING, CHEMICAL

Processes Pub Date : 2025-09-17 DOI:10.3390/pr13092960

Peng Zhan, Yuxin Tan, Hui Wang, Jin Liu, Lishu Shao, Zhiping Wu

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引用次数: 3

Abstract

Surfactants demonstrate considerable potential in enzymatic saccharification at high solids loading (ESHSL). In this paper, the effects of the non-ionic surfactant Tween 80 on enzymatic saccharification of Avicel and steam-exploded poplar (SEP) at high solid loading were studied. The results showed that under the fed-batch conditions of 15.0% solid loading, 20 FPU/g glucan, and 1.0% Tween 80, the maximum enzymatic saccharification rate of Avicel and SEP achieved was 65.4% (128.2 g/L glucose) and 86.4% (93.9 g/L glucose), respectively. Moreover, Tween 80 improved the rheological properties of ESHSL slurry of SEP, especially for the fed-batch model, reducing the complex viscosity, shear stress, and storage modulus. Furthermore, cellulase adsorption assays, SDS-PAGE, Rose Bengal staining, and Zeta potential analysis demonstrated that Tween 80 reduced non-productive adsorption of cellulase (particularly β-glucosidase) on lignin through hydrophobic interactions. All these findings contribute to establishing a foundation for subsequent investigative efforts within the discipline.

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非离子表面活性剂Tween 80对高固载条件下杨树和蒸汽爆炸树酶解糖化的影响

表面活性剂在高固体负荷（ESHSL）的酶催化糖化中显示出相当大的潜力。研究了非离子表面活性剂Tween 80在高固载条件下对Avicel和蒸汽爆炸杨树（SEP）酶解糖化的影响。结果表明，在固载量15.0%、葡聚糖20 FPU/g、Tween 80 1.0%的补料条件下，Avicel和SEP的最大酶解率分别为65.4% （128.2 g/L葡萄糖）和86.4% （93.9 g/L葡萄糖）。此外，Tween 80改善了SEP ESHSL料浆的流变性能，降低了复合粘度、剪切应力和储存模量，特别是对于进料批模型。此外，纤维素酶吸附实验、SDS-PAGE、Rose Bengal染色和Zeta电位分析表明，Tween 80通过疏水相互作用减少了纤维素酶（特别是β-葡萄糖苷酶）对木质素的非生产吸附。所有这些发现都有助于为该学科的后续调查工作奠定基础。

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来源期刊

Processes Chemical Engineering-Bioengineering

CiteScore

5.10

自引率

11.40%

发文量

2239

审稿时长

14.11 days

期刊介绍： Processes (ISSN 2227-9717) provides an advanced forum for process related research in chemistry, biology and allied engineering fields. The journal publishes regular research papers, communications, letters, short notes and reviews. Our aim is to encourage researchers to publish their experimental, theoretical and computational results in as much detail as necessary. There is no restriction on paper length or number of figures and tables.