Investigating Microtubule-Associated Protein 2 in the Study of Postoperative Delirium.

Delirium (Bielefeld, Germany) Pub Date : 2026-01-01 Epub Date: 2026-01-27 DOI:10.56392/001c.155608

Tina McKay, Occam Kelly Graves, Anna Toombs, Christopher Simon, Oluwaseun Akeju

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Abstract

Background: This study explored potential associations between microtubule-associated protein 2 (MAP2), as a marker of neuronal stress, and postoperative delirium.

Methods: Custom single-molecule immunoassays were used to measure MAP2 in serum collected from two small cohorts of older adults undergoing major cardiac surgery. An in vitro study of differentiated SH-SY5Y cells derived from a neuroblastoma cell line was performed to assess MAP2 secretion following exposure to acute glutamate stimulation.

Results: Patients who experienced postoperative delirium within 3 days of surgery had increased MAP2 serum levels on postoperative day 1. Perioperative blood sampling revealed an increase in circulating MAP2 at the end of cardiopulmonary bypass that was sustained up to postoperative day 3 in patients who developed delirium. Differentiated SH-SY5Y cells exposed to high-dose glutamate showed increased MAP2 in conditioned media independent of changes in total cytosolic protein levels suggesting that extracellular stimuli may promote acute secretion of MAP2 without inducing cell rupture.

Conclusion: Further studies exploring the MAP family as biomarkers of cognitive changes following surgery are warranted.

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微管相关蛋白2在术后谵妄研究中的作用。

背景：本研究探讨了作为神经元应激标志物的微管相关蛋白2 （MAP2）与术后谵妄之间的潜在关联。方法：采用定制的单分子免疫测定法测量两组接受大心脏手术的老年人血清中的MAP2。一项来自神经母细胞瘤细胞系的分化SH-SY5Y细胞的体外研究评估了暴露于急性谷氨酸刺激后MAP2的分泌。结果：术后3天出现谵妄的患者术后第1天血清MAP2水平升高。围手术期血液采样显示，在发生谵妄的患者中，循环MAP2在体外循环结束时持续到术后第3天。暴露于高剂量谷氨酸的分化SH-SY5Y细胞在条件培养基中显示MAP2增加，而不依赖于胞质总蛋白水平的变化，这表明细胞外刺激可能促进MAP2的急性分泌，而不诱导细胞破裂。结论：进一步研究MAP家族作为手术后认知变化的生物标志物是有必要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Delirium (Bielefeld, Germany)

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