Immunohistochemical localization of inhibin in porcine and bovine ovaries.

Abstract

The immunohistochemical localization of inhibin in porcine and bovine ovaries was studied, using monoclonal antibodies to bovine follicular fluid 32K inhibin (bFF 32K inhibin) and a polyclonal antiserum to porcine follicular fluid 32K inhibin (pFF 32K inhibin). In order to obtain a precise immunohistochemical staining, various fixations were tested with an immunoblotting procedure. Acetone fixation followed by celloidin embedding proved to be most appropriate for the immunohistochemical study of inhibin. A consistent pattern emerged in all sections prepared from porcine and bovine ovaries. Granulosa cells were specifically stained by the antibodies, and especially the cells constituting the inner layer were more intensely stained than the cells close to the theca.