A comparison of three anti-double stranded DNA antibody assays on sera from SLE and other diseases.

Diagnostic immunology Pub Date : 1986-01-01

M C Clarke, R Carr, N M Burdash, Z Y Chen, S K Ainsworth

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Abstract

Systemic lupus erythematosus (SLE) is a multisystem disorder accompanied by a diverse spectrum of serum autoantibodies. Antibodies to double stranded DNA (dsDNA) are considered to be the most specific marker for this disease. In this study the results obtained from three different assays for dsDNA are compared: an indirect fluorescence antibody assay (IFA), a radioimmunoassay (RIA) and, an enzyme-linked immunosorbent assay (ELISA), on 57 SLE sera and 28 Sera from other disorders. Correlation of these anti-DNA results are made with C3, C4, and antinuclear antibody (ANA) titers. Our results show the IFA assay is the most sensitive and the least specific of the three tests. The RIA was found to be the most specific and was approximately as sensitive as the ELISA. We also found significant inverse correlations between anti-dsDNA levels and circulating complement levels among SLE sera for all three assays. ANA titers were significantly correlated with all anti-dsDNA assays as well. However, these anti-dsDNA assays show only modest differences explainable by numerous mechanisms. Hence, a clearly superior anti-dsDNA method does not emerge from our study.

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SLE与其他疾病血清抗双链DNA抗体测定的比较。

系统性红斑狼疮(SLE)是一种多系统疾病，伴有不同谱的血清自身抗体。双链DNA (dsDNA)抗体被认为是该病最特异的标志物。在这项研究中，对57例SLE血清和28例其他疾病血清的dsDNA进行了间接荧光抗体测定(IFA)、放射免疫测定(RIA)和酶联免疫吸附测定(ELISA)，比较了三种不同测定方法获得的结果。这些抗dna结果与C3、C4和抗核抗体(ANA)滴度相关。我们的结果表明，IFA法是最敏感的，特异性最低的三个测试。发现RIA是最特异的，其敏感性与ELISA近似。我们还发现，在所有三种检测中，SLE血清中抗dsdna水平与循环补体水平之间存在显著的负相关。ANA滴度也与所有抗dsdna检测结果显著相关。然而，这些抗dsdna分析显示只有适度的差异，可以解释许多机制。因此，我们的研究并没有产生一种明显优越的抗dsdna方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Diagnostic immunology

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