Rapid dot-immunobinding for detecting antibodies to Toxoplasma gondii in human serum.

Abstract

A modified dot-immunobinding assay is described for the rapid, specific and reliable screening of human sera for Toxoplasma gondii immunity status. This simple, rapid and sensitive assay proved to be a practical diagnostic technique since it can be performed in 3 hours employing a small amount of antigen (0.3 micrograms/microliters).