The relative resistance of lymphokine activated killer cells to suppression by prostaglandins and glucocorticoids

T. Imir , W. Sibbitt, A. Bankhurst
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引用次数: 17

Abstract

The possibility that lymphokine-activated killer (LAK) cells versus spontaneous natural killer (NK) cells show relative resistance to the suppressive effects of the immunoregulatory molecules prostaglandin E2 (PGE2) and dexamethasone (DMO) was investigated. LAK cells were produced in vitro by the incubation of human peripheral mononuclear cells (PBMC) for three days in the presence of interleukin-2 (IL-2). Cytotoxicity of NK and LAK cells were measured by conventional 4 hour Cr51 release assays using K562 and Daudi target cells. LAK cells were relatively resistant to suppression by PGE2. For example, NK cytotoxicity was significantly suppressed by 10−6 M PGE2. In contrast, LAK cells required a 30 to 100 higher concentration of PGE2 according to the target used to achieve similar suppression. Likewise, a differential resistance to DMO was seen. NK cells were significantly suppressed by 10-3M DMO while a 1000 fold higher concentration was needed for similar suppression of LAK cytotoxicity. Overall, the results show that LAK cells are relatively resistant to immunoregulatory suppressive factors.

淋巴因子激活的杀伤细胞对前列腺素和糖皮质激素抑制的相对抗性
研究了淋巴因子激活的杀伤细胞(LAK)与自发自然杀伤细胞(NK)对免疫调节分子前列腺素E2 (PGE2)和地塞米松(DMO)的抑制作用表现出相对抗性的可能性。在白细胞介素-2 (IL-2)存在下,人外周血单核细胞(PBMC)体外培养3天,产生LAK细胞。以K562和Daudi为靶细胞,采用常规的4小时Cr51释放法测定NK和LAK细胞的细胞毒性。LAK细胞相对抵抗PGE2的抑制。例如,10−6 M PGE2显著抑制NK细胞毒性。相比之下,LAK细胞需要的PGE2浓度比用于实现类似抑制的靶标高30至100。同样,对DMO有不同的抗性。10-3M DMO对NK细胞有明显的抑制作用,而对LAK细胞毒性的抑制作用需要高出1000倍的浓度。综上所述,LAK细胞对免疫调节抑制因子具有相对的抗性。
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