Semi-synthesis of sodium copper chlorophyllin from Chlorella vulgaris cultivated under continuous photoperiod and sequential extraction of fatty acids and exopolysaccharides

IF 4.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2026-03-01 Epub Date: 2026-01-31 DOI:10.1016/j.fbp.2026.01.025

Jamila Sueira de Jesus Silva , Fábio Alexandre Chinalia , Carolina Oliveira de Souza , Denilson de Jesus Assis , Jania Betânia Alves da Silva , Gabriele Marques dos Santos , Fernanda Teixeira Cruz , Ravena Maria de Almeida Medeiros , Paulo Vitor França Lemos , Lucas Guimarães Cardoso

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引用次数: 0

Abstract

This study aimed to produce and characterize sodium copper chlorophyllin (SCC) from the biomass of Chlorella vulgaris under 12-hour and 24-hour light photoperiods and to analyze the composition of lipids, fatty acids, and pigments in the resulting biomass. SCC production yielded 310 mg g⁻¹ under the 12-hour photoperiod (FT12) and 300 mg g⁻¹ under the 24-hour photoperiod (FT24). The SCC exhibited absorbance band, functional groups, and primary SCC derivatives, comparable to commercial SCC. LC-MS identified the main SCC derivatives Cu-chlorin p6, Cu-rhodin g7, and Cu-chlorin e6. SCC semi-synthesis can be improved by omitting the overnight step, reducing production time. Sequencial exctraction yielded lipids (10.54 % and 12.02 % in FT12 and FT24, respectively), with major fatty acids identified as C18:1 and C16:0 before saponification and C16:0 and C18:3n3 after saponification. Additionally, extracellular polymeric substances (EPS) were obtained. The developed bioprocess enables the semi-synthesis of SCC while recovering bioproducts of high added value.

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连续光周期培养小球藻半合成叶绿素铜钠及脂肪酸和胞外多糖的顺序提取

本研究旨在利用普通小球藻（Chlorella vulgaris）在12小时和24小时光照周期下的生物量生产和表征叶绿素铜钠（SCC），并分析其脂质、脂肪酸和色素的组成。12小时光周期（FT12）下SCC产量为310 mg g−1,24小时光周期（FT24）下SCC产量为300 mg g−1。SCC具有与商品SCC相当的吸光度带、官能团和初级SCC衍生物。LC-MS鉴定了SCC的主要衍生物Cu-chlorin p6、Cu-rhodin g7和Cu-chlorin e6。通过省略隔夜步骤，减少生产时间，可以改善SCC半合成。顺序提取得到的脂质在FT12和FT24中分别为10.54 %和12.02 %，主要脂肪酸在皂化前鉴定为C18:1和C16:0，皂化后鉴定为C16:0和C18:3n3。此外，还获得了细胞外聚合物（EPS）。开发的生物工艺使SCC的半合成成为可能，同时回收高附加值的生物产品。

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.