Differential expression of CD74 and C1QB in jaw versus long bone osteosarcoma: Insights from animal models, public datasets and clinical cohorts

IF 3.5 2区医学 Q2 Medicine

Journal of Bone Oncology Pub Date : 2026-04-01 Epub Date: 2026-01-23 DOI:10.1016/j.jbo.2026.100742

Lalan Zhang , Jiaoyan Liu , Shengjie Shao , Xiang Liu , Liqin Zhang , Weihong Wang

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引用次数: 0

Abstract

Objective

This study aimed to investigate the differential expression of macrophage-related factors between jaw and long bone osteosarcoma, thereby providing potential candidates for future functional and mechanistic research.

Methods

Twenty-four Sprague-Dawley (SD) rats were randomly divided into control, jaw osteosarcoma, and long bone osteosarcoma groups. UMR106 cells were implanted subcutaneously in nude mice, then tumors were transplanted into rat jaw and tibial bone marrow to establish osteosarcoma models. Bone changes were observed using Micro-CT, while histological changes were examined with H&E and Masson’s trichrome staining. Transcriptome sequencing identified differentially expressed genes (DEGs), with the top three genes selected using Cytohubba software. Survival analysis was performed using the GEPIA database. A retrospective analysis was conducted on jaw osteosarcoma patients and long bone osteosarcoma patients from November 2014 to June 2023. Finally, immunohistochemical staining validated the expression levels of relevant proteins in both animal and clinical tissue samples.

Results

Micro-CT revealed significant bone destruction in both osteosarcoma models. Histological analysis revealed high pleomorphism in osteosarcoma cells, predominantly spindle and polygonal shapes, with evident pathological mitosis. Transcriptome sequencing identified 414 DEGs, including the top three: RT1-Da, CD74, and C1QB. Survival analysis showed high expression of CD74 and C1QB correlated positively with overall survival in patients with osteosarcoma. Immunohistochemistry demonstrated higher CD74 and C1QB expression in jaw osteosarcoma compared to long bone osteosarcoma in both rats and humans (P < 0.05).

Conclusion

The expression levels of CD74 and C1QB were significantly higher in jaw osteosarcoma than in long bone osteosarcoma, suggesting that this differential expression may have clinical relevance for patient survival and warrants further investigation.

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CD74和C1QB在颌骨与长骨骨肉瘤中的差异表达：来自动物模型、公共数据集和临床队列的见解

目的探讨巨噬细胞相关因子在颌骨和长骨骨肉瘤中的表达差异，为未来的功能和机制研究提供潜在的候选物。方法将24只SD大鼠随机分为对照组、颌骨骨肉瘤组和长骨骨肉瘤组。裸鼠皮下植入UMR106细胞，将肿瘤植入大鼠颌骨和胫骨骨髓，建立骨肉瘤模型。Micro-CT观察骨变化，H&；E和Masson’s三色染色观察组织学变化。转录组测序鉴定差异表达基因（deg），使用Cytohubba软件选择前三个基因。使用GEPIA数据库进行生存分析。回顾性分析2014年11月至2023年6月的颌骨骨肉瘤和长骨骨肉瘤患者。最后，免疫组织化学染色验证了相关蛋白在动物和临床组织样本中的表达水平。结果显微ct显示两种骨肉瘤模型均有明显的骨破坏。组织学分析显示骨肉瘤细胞高度多形性，以梭形和多角形为主，有明显的病理性有丝分裂。转录组测序鉴定出414个deg，包括前三个：RT1-Da， CD74和C1QB。生存分析显示，CD74和C1QB的高表达与骨肉瘤患者的总生存呈正相关。免疫组化结果显示，大鼠和人颌骨骨肉瘤中CD74和C1QB的表达均高于长骨骨肉瘤（P < 0.05）。结论CD74和C1QB在颌骨骨肉瘤中的表达水平明显高于长骨骨肉瘤，提示这种差异表达可能与患者生存有临床相关性，值得进一步研究。

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来源期刊

Journal of Bone Oncology ONCOLOGY-

CiteScore

7.20

自引率

2.90%

发文量

审稿时长

34 days

期刊介绍： The Journal of Bone Oncology is a peer-reviewed international journal aimed at presenting basic, translational and clinical high-quality research related to bone and cancer. As the first journal dedicated to cancer induced bone diseases, JBO welcomes original research articles, review articles, editorials and opinion pieces. Case reports will only be considered in exceptional circumstances and only when accompanied by a comprehensive review of the subject. The areas covered by the journal include: Bone metastases (pathophysiology, epidemiology, diagnostics, clinical features, prevention, treatment) Preclinical models of metastasis Bone microenvironment in cancer (stem cell, bone cell and cancer interactions) Bone targeted therapy (pharmacology, therapeutic targets, drug development, clinical trials, side-effects, outcome research, health economics) Cancer treatment induced bone loss (epidemiology, pathophysiology, prevention and management) Bone imaging (clinical and animal, skeletal interventional radiology) Bone biomarkers (clinical and translational applications) Radiotherapy and radio-isotopes Skeletal complications Bone pain (mechanisms and management) Orthopaedic cancer surgery Primary bone tumours Clinical guidelines Multidisciplinary care Keywords: bisphosphonate, bone, breast cancer, cancer, CTIBL, denosumab, metastasis, myeloma, osteoblast, osteoclast, osteooncology, osteo-oncology, prostate cancer, skeleton, tumour.