Bacterial Diversity of Deep Carious Lesions Analysed by Next-Generation Sequencing Approaches Associated With Different Pulp Diagnoses: A Systematic Review

IF 7.1 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International endodontic journal Pub Date : 2026-04-09 Epub Date: 2026-02-12 DOI:10.1111/iej.70114

Giorgos N. Tzanetakis, Maria-Eleni Markou, Despina Koletsi, Venkateshbabu Nagendrababu, Henry F. Duncan

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引用次数: 0

Abstract

Aim

This systematic review aimed to synthesise evidence on the microbiome of deep or extremely deep caries associated with a range of different pulp conditions, by analysing studies using next-generation sequencing (NGS) approaches.

Methodology

The review reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines and protocol was registered prospectively at the Open Science Framework (https://osf.io/vnwjh/). A literature search was carried out in the following databases: MEDLINE via PubMed, Scopus, Cochrane Database for Systematic Reviews (CDSR), Web of Science, the clinicaltrials.gov, and the Open Science Framework, in English language without time restriction. The last search was performed on May 1st 2025. An additional online search of the four major journals in the field of endodontics (International Endodontic Journal, Journal of Endodontics, Australian Endodontic Journal and the European Endodontic Journal) was also carried out independently starting from January 1st, 2010 to May 1st 2025. Only original research articles that used an NGS approach and investigated the microbiome of deep or extremely deep caries associated with different pulp diagnoses, assessed preoperatively, were included. The Johanna Brigg's Institute (JBI's) Critical Appraisal Instrument for Studies Reporting Prevalence Data was used to assess the risk of bias of included studies.

Results

Among 1914 articles initially identified, four were considered eligible for inclusion and further assessed for certain parameters. The overall risk of bias of the three and one included studies was characterised as ‘unclear’ and ‘high’. Firmicutes were the most prevalent phylum in all cases. In cases of irreversible pulpitis, Firmicutes prevalence increased compared with cases of normal pulp and reversible pulpitis, while the prevalence of Actinobacteria, Proteobacteria and Bacteroidetes decreased. Lactobacillus was the most abundant genus in all cases, with its abundance in cases of symptomatic irreversible pulpitis increasing substantially.

Conclusions

The NGS studies evaluated in this review create a preliminary, but comprehensive map of the microbiome of deep caries associated with a range of pulpal diagnoses. Α shift in microbiome as the asymptomatic cases become progressively symptomatic seems to occur, which may be relevant to management of deep caries in terms of complete or selective caries removal.

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用与不同牙髓诊断相关的新一代测序方法分析深部龋齿病变的细菌多样性：一项系统综述。

目的：本系统综述旨在通过分析使用下一代测序（NGS）方法的研究，合成与一系列不同牙髓状况相关的深部或极深部龋微生物组的证据。方法：根据系统评价和荟萃分析2020指南和方案的首选报告项目报告的综述在开放科学框架（https://osf.io/vnwjh/）前瞻性注册。文献检索：MEDLINE通过PubMed、Scopus、Cochrane Database for Systematic Reviews （CDSR）、Web of Science、the clinicaltrials.gov和the Open Science Framework进行检索，检索语言为英文，无时间限制。最后一次搜索是在2025年5月1日。另外，从2010年1月1日至2025年5月1日，独立进行了牙髓学领域四大期刊（International Endodontic Journal, Journal of endodontics， Australian Endodontic Journal和European Endodontic Journal）的在线检索。仅纳入了使用NGS方法调查与不同牙髓诊断相关的深部或极深部龋的微生物组的原始研究文章，并进行了术前评估。使用约翰娜布里格研究所（JBI）报告患病率数据的研究关键评估工具来评估纳入研究的偏倚风险。结果：在最初确定的1914篇文章中，有4篇被认为符合纳入条件，并对某些参数进行了进一步评估。纳入的三个和一个研究的总体偏倚风险被描述为“不清楚”和“高”。厚壁菌门是所有病例中最常见的门。在不可逆性牙髓炎病例中，与正常牙髓和可逆性牙髓炎病例相比，厚壁菌门的患病率增加，而放线菌门、变形菌门和拟杆菌门的患病率下降。乳酸菌是所有病例中最丰富的属，在症状性不可逆牙髓炎病例中其丰度显著增加。结论：本综述中评估的NGS研究创建了与一系列牙髓诊断相关的深部龋微生物组的初步但全面的图谱。Α随着无症状病例逐渐出现症状，微生物组似乎发生了变化，这可能与完全或选择性去除深部龋的管理有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International endodontic journal 医学-牙科与口腔外科

CiteScore

10.20

自引率

28.00%

发文量

195

审稿时长

4-8 weeks

期刊介绍： The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.