Comparative analysis of O6-methylguanine methyltransferase activity and cellular sensitivity to alkylating agents in cell strains derived from a variety of animal species

Mutation Research/DNA Repair Reports Pub Date : 1987-09-01 DOI:10.1016/0167-8817(87)90073-3

Mituo Ikenaga , Tohru Tsujimura , Hae-Ryong Chang , Chikau Fujio , Yang-Pei Zhang , Kanji Ishizaki , Hiroko Kataoka , Akihiro Shima

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引用次数: 27

Abstract

Using 26 cultured cell lines derived from 17 different animal species, we have measured both the activity of O⁶-methylguanine (O⁶-MeG) methyltransferase (MT) in cell extracts and the sensitivity of the strains to the lethal effects of the alkylating agents, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) and 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU). The MT activity was assayed by measuring the amount of ³H radioactivity transferred from methyl-[³H]-labeled O⁶-MeG in DNA to acceptor protein molecules in the extracts. In all the 21 mammalian cell strains, lethal sensitivity to ACNU as measured by colony-forming ability correlated well with cellular MT activity, indicating that the major lethal ACNU damage is reparable by the MT. On the other hand, MNNG sensitivity did not necessarily correlate with the MT activity.

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不同动物来源细胞株o6 -甲基鸟嘌呤甲基转移酶活性及细胞对烷基化剂敏感性的比较分析

利用来自17种不同动物的26株培养细胞系，测定了细胞提取物中o6 -甲基鸟嘌呤(O6-MeG)甲基转移酶(MT)的活性，以及菌株对烷基化剂n -甲基-n ' -硝基-n -亚硝基胍(MNNG)和1-(4-氨基-2-甲基-5-嘧啶基)甲基-3-(2-氯乙基)-3-亚硝基脲(ACNU)致死作用的敏感性。通过测量DNA中甲基[3H]标记的O6-MeG向提取物中受体蛋白分子转移的3H放射性量来测定MT活性。在所有21种哺乳动物细胞株中，通过集落形成能力测量的ACNU致死敏感性与细胞MT活性相关良好，表明主要的ACNU致死损伤可以通过MT修复。另一方面，MNNG敏感性与MT活性并不一定相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mutation Research/DNA Repair Reports

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