Modulation of class-II antigen expression in human thyroid epithelial cell cultures.

B E Wenzel, H Arnholdt, S Grammerstorf, R Gutekunst, P C Scriba
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引用次数: 3

Abstract

The modulation of HLA-D expression of thyroid epithelial cells (TEC) was studied in vitro by means of immunofluorescence. Under serum-free culture conditions, TSH and TSH-receptor antibodies induce HLA-D on TECs derived from GD-patients. Serum-free culture conditions provide a higher availability of TSH-receptors by a 'right side right' polarity of the cellular morphology. There was no evidence for IFN-gamma producing cell contaminations on GD-TECs. TSH in contrast to IFN-gamma does not induce HLA-DQ on TECs. HLA-DQ is not displayed by spontaneously class-II antigen expressing GD-TECs. Methimazole as well as perchlorate do not suppress HLA-D expression of TECs.

ii类抗原在人甲状腺上皮细胞培养中的表达调控。
采用免疫荧光法研究了体外甲状腺上皮细胞(TEC) HLA-D表达的调节作用。在无血清培养条件下,TSH和TSH受体抗体在gd患者的tec上诱导HLA-D。无血清培养条件通过细胞形态的“右侧右”极性提供更高的tsh受体可用性。没有证据表明在gd - tec上产生ifn - γ的细胞污染。与ifn - γ相比,TSH不会诱导tec上的HLA-DQ。HLA-DQ不被自发表达的ii类抗原的gd - tec所显示。甲巯咪唑和高氯酸盐不抑制TECs的HLA-D表达。
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