Effect of 16,16-dimethyl prostaglandin E2 on mucus glycoprotein biosynthesis in rat stomach and duodenal glands

Thea Jentjens, Ger J. Strous
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引用次数: 8

Abstract

The present study describes the effect of 16,16-dimethyl prostaglandin E2 (16,16-dmPGE2) on mucus glycoprotein biosynthesis in rat stomach and duodenal glands. After in vivo treatment with 16,16-dmPGE2 (10 μg/kg subcutaneously) for 1 h, the incorporation rate of [3H]galactose, [3H]glucosamine, and [3H]serine in the ex vivo vascularly perfused stomach was determined by light microscopic autoradiography.

As was previously found by us for the surface mucous cells in the fundus of 16,16-dmPGE2-treated rats, the incorporation of [3H]galactose and [3H]glucosamine (indicative of mucus glycoprotein synthesis) in the isthmus was increased two- to fourfold. Small if any increases were detected in the mucous cells near the base of the glands of the fundus (neck cells), the mucous cells in the antrum and the mucous cells in the duodenal glands. Total protein synthesis as measured by [3H]serine incorporation was not increased in any of these cells.

We conclude that 16,16-dmPGE2 has different effects on mucus glycoprotein biosynthesis in various regions of the rat stomach. Increased biosynthesis in the fundus points to a role for mucus in the prostaglandin-induced protection of the gastric mucosa against injury.

16,16-二甲基前列腺素E2对大鼠胃和十二指肠腺粘液糖蛋白生物合成的影响
本研究描述了16,16-二甲基前列腺素E2 (16,16- dmpge2)对大鼠胃和十二指肠腺粘液糖蛋白生物合成的影响。16、16- dmpge2 (10 μg/kg)在体内皮下作用1 h后,用光镜放射自显影法测定[3H]半乳糖、[3H]氨基葡萄糖和[3H]丝氨酸在体外血管灌注胃中的掺入率。正如我们之前在16,16- dmpge2处理大鼠眼底表面粘膜细胞中发现的那样,峡部[3H]半乳糖和[3H]氨基葡萄糖(表明粘液糖蛋白合成)的掺入增加了2至4倍。在眼底腺体底部附近的黏液细胞(颈部细胞)、胃窦的黏液细胞和十二指肠腺体的黏液细胞中检测到少量的(如果有的话)增加。用[3H]丝氨酸掺入法测定的总蛋白合成在这些细胞中均未增加。我们认为16,16- dmpge2对大鼠胃不同区域粘液糖蛋白的生物合成有不同的影响。眼底生物合成的增加表明黏液在前列腺素诱导的胃粘膜保护中起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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