Multilocus linkage analysis of markers located on short arm of chromosome 11.

Abstract

The computer program package LINKAGE (Lathrop e t a l , 1984; 1985) w a s used f o r t h e ana lyses . I n a f i r s t s t e p we performed two p o i n t a n a l y s i s u s ing t h e programs M L I N K , which c a l c u l a t e s l i ke l ihoods f o r va r ious va lues of recombination f r a c t i o n , and I L I N K which provides maximum l i k e l i h o o d e s t ima tes of recombina t i o n f r a c t i o n s , a l lowing f o r s e x d i f f e rence . A prov i s iona l o r d e r was thus obta ined . Then t h r e e p o i n t a n a l y s i s w a s per for med us ing I L I N K . We used t h e s t a t e g y suggested by Lathrop e t a1 (1984, 1985) : assuming no c o n s t r a i n t between the t h r e e recomb i n a t i o n f r a c t i o n s e l , B2, O 3 which is equ iva len t t o a l low f o r i n t e r f e r e n c e wi th u n r e s t r i c t e d c o e f f i c i e n t of coincidence -, t he e s t ima tes of e l , e2, e y i e l d d i r e c t l y the most l i k e l y o r d e r of l o c i , whatever t h e i n i t i a l o r d e r given i n the a n a l y s i s . 3