Cleavage Under Targets and Tagmentation (CUT&Tag) Protocol for Plants.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2026-01-01 DOI:10.1007/978-1-0716-4972-5_3

Xiao-Yuan Tao, Jia-Wei Wang

引用次数: 0

Abstract

CUT&Tag (Cleavage Under Targets and Tagmentation) is a powerful method for chromatin profiling based on an enzyme-tethering strategy. Compared with chromatin immunoprecipitation (ChIP) assay, CUT&Tag requires smaller amount of cell/nuclei input and generates data with higher signal-to-noise ratio, which has been widely applied in profiling histone modifications and protein-DNA interactions. Here, we describe a detailed protocol for bulk-cell CUT&Tag in plants and briefly introduced other derivative CUT&Tag methods.

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植物靶下切割和标记（CUT&Tag）协议。

CUT&Tag（目标下切割和标记）是一种基于酶系固策略的染色质分析的强大方法。与染色质免疫沉淀（ChIP）法相比，CUT&Tag法需要更少的细胞/细胞核输入，产生的数据具有更高的信噪比，在组蛋白修饰和蛋白质- dna相互作用分析中得到了广泛的应用。在此，我们描述了植物体细胞CUT&Tag的详细协议，并简要介绍了其他衍生的CUT&Tag方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.