Screening for colon carcinogens--a new strategy.

Molecular toxicology Pub Date : 1987-04-01
J A Heddle, H K Kaul, J D Gingerich, D B Couch
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Abstract

The major defect of in vivo assays for mutagenic carcinogens may be tissue specificity: a cancer bioassay of a single tissue would not be expected to detect all carcinogens, so the failure of a genetic assay in a single tissue to detect all carcinogens should not be surprising. In the search for an environmental carcinogen responsible for a specific cancer in a particular population, however, it may be that tissue specificity can be advantageous. Assays for genotoxicity directly in the target cells may have higher success rates with fewer false positives than assays in tissues of convenience. For example, to facilitate the search for one or more dietary carcinogens responsible for the high rate of colon cancer in North America, assays for genotoxicity in the target cells themselves, the colonic epithelium, may be useful. To this end we have investigated assays for three different endpoints: nuclear anomalies, sister chromatid exchanges, and gene mutations. Our experience may prove useful for others considering a similar strategy.

筛查结肠癌——一种新策略。
体内诱变致癌物检测的主要缺陷可能是组织特异性:单个组织的癌症生物检测不可能检测到所有致癌物,因此单个组织的基因检测无法检测到所有致癌物不应该令人惊讶。然而,在寻找导致特定人群中特定癌症的环境致癌物时,组织特异性可能是有利的。直接在靶细胞中测定遗传毒性比在方便的组织中测定具有更高的成功率和更少的假阳性。例如,为了寻找一种或多种导致北美地区结肠癌高发病率的膳食致癌物,对目标细胞(结肠上皮细胞)本身进行遗传毒性测定可能是有用的。为此,我们研究了三个不同终点的检测方法:核异常、姐妹染色单体交换和基因突变。我们的经验可能对其他考虑类似策略的国家有用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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