Quantification of aflatoxins in animal feed in Punjab, Pakistan, using HPLC-FLD and confirmation by LC-MS/MS.

IF 2.5 3区农林科学 Q2 CHEMISTRY, APPLIED

Food additives & contaminants. Part B, Surveillance Pub Date : 2026-03-01 Epub Date: 2025-12-09 DOI:10.1080/19393210.2025.2576912

Mateen Abbas, Abdul Muqeet Khan, Zara Hussain, Attia Abdullah

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引用次数: 0

Abstract

Animal feedstuffs are prone to fungal contamination due to their protein content and high moisture levels at harvest and storage, often leading to mycotoxin production. This study investigated the occurrence of aflatoxins in animal feed and the feed ingredients cottonseed cake and maize oil cake, collected from livestock farms in Okara, Punjab, Pakistan (2021-2022). Aflatoxins were extracted with an acetonitrile - water mixture, purified with Mycosep columns, quantified by HPLC with fluorescence detection and confirmed by mass spectrometry. Aflatoxins were detected in 74% of the animal feed samples, in 87% of the cottonseed cake samples and in 63% of the maize oil cake samples. Notably, 33% of animal feed and 70% of oilseed cake samples exceeded the maximum limit of 20 µg/kg. These findings revealed a high level of aflatoxin contamination, particularly in oilseed cakes, with statistically significant results (p < 0.05), highlighting potential animal health risks.

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HPLC-FLD法测定巴基斯坦旁遮普省动物饲料中黄曲霉毒素含量，LC-MS/MS法确认。

动物饲料由于其蛋白质含量和收获和储存时的高水分水平，容易受到真菌污染，往往导致真菌毒素的产生。本研究调查了在巴基斯坦旁遮普省奥卡拉（Okara）的牲畜养殖场采集的动物饲料及其原料棉籽饼和玉米油饼中黄曲霉毒素的含量（2021-2022年）。黄曲霉毒素用乙腈-水混合物提取，用Mycosep色谱柱纯化，用高效液相色谱荧光检测定量，并用质谱法确证。在74%的动物饲料样品、87%的棉籽饼样品和63%的玉米油饼样品中检测到黄曲霉毒素。值得注意的是，33%的动物饲料和70%的油籽饼样品超过了20µg/kg的最大限值。这些调查结果显示黄曲霉毒素污染程度很高，特别是在油籽饼中，统计结果具有显著意义

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food additives & contaminants. Part B, Surveillance 农林科学-毒理学

CiteScore

5.30

自引率

10.30%

发文量

审稿时长

2.7 months

期刊介绍： Food Additives & Contaminants: Part B publishes surveillance data indicating the presence and levels of occurrence of designated food additives, residues and contaminants in foods, food supplements and animal feed. Data using validated methods must meet stipulated quality standards to be acceptable and must be presented in a prescribed format for subsequent data-handling. Food Additives & Contaminants: Part B restricts its scope to include certain classes of food additives, residues and contaminants. This is based on a goal of covering those areas where there is a need to record surveillance data for the purposes of exposure and risk assessment. The scope is initially restricted to: Additives - food colours, artificial sweeteners, and preservatives; Residues – veterinary drug and pesticide residues; Contaminants – metals, mycotoxins, phycotoxins, plant toxins, nitrate/nitrite, PCDDs/PCFDs, PCBs, PAHs, acrylamide, 3-MPCD and contaminants derived from food packaging. Readership: The readership includes scientists involved in all aspects of food safety and quality and particularly those involved in monitoring human exposure to chemicals from the diet. Papers reporting surveillance data in areas other than the above should be submitted to Part A . The scope of Part B will be expanded from time-to-time to ensure inclusion of new areas of concern.