The first complete mitochondrial genome characterization of Lutjanus fulvus (Perciformes: Lutjanidae) and phylogenetic analysis within the Lutjanidae family

IF 1.3 3区生物学 Q2 ZOOLOGY

Zoologischer Anzeiger Pub Date : 2026-01-01 Epub Date: 2025-12-04 DOI:10.1016/j.jcz.2025.12.001

Luxiu Gao , Yifan Liu , Faiz Muhammad , Taobo Feng , Mingzhe Han , Chi Zhang , Bingjian Liu

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引用次数: 0

Abstract

In this study, we obtained the complete mitogenome of Lutjanus fulvus from a specimen collected in Pakistani waters (63.95°E, 25.30°N) by utilizing high-throughput sequencing technology, then we characterized it and downloaded the complete mitogenomes of closely related species to constructed phylogenetic trees based on 13 protein-coding genes (PCGs) using Bayesian Inference (BI) methods. The complete mitogenome of L. fulvus is a typical closed circular double-stranded DNA molecule, was 16 493 bp in length, with a relatively low G content (16.2 %) and a high AT pronounced bias (52.8 %). The genome comprises 37 genes: 22 tRNAs, two rRNAs, 13 PCGs, and one putative control region (D-loop). Among the 13 PCGs, four types of stop codons (TAA, TAG, AGA, T--) are observed, and all genes, except for COX1, which starts with GTG, begin with the ATG start codon. The BI trees exhibited consistent topologies, indicating close relationships with Lutjanus vitta and Lutjanus ophuysenii. This study provides valuable genomic insights into the mitogenome of L. fulvus, enhancing our understanding of phylogenetic relationships within the Lutjanidae family and offering a fundamental reference for further exploration of mitogenomes in the Lutjanus genus. Furthermore, these data contribute new molecular evidence and theoretical foundations for the classification, genetic diversity studies, and the conservation and management of L. fulvus.

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首个全线粒体基因组鉴定及系统发育分析

本研究利用高通量测序技术，从巴基斯坦海域（63.95°E, 25.30°N）采集了黄颡鱼（Lutjanus fulvus）的有丝分裂全基因组，对其进行了鉴定，并下载了近缘种的有丝分裂全基因组，利用贝叶斯推理（Bayesian Inference， BI）方法构建了基于13个蛋白编码基因（PCGs）的系统发育树。L. fulvus全长16 493 bp，是一个典型的封闭环状双链DNA分子，G含量较低（16.2%），AT明显偏倚较高（52.8%）。基因组由37个基因组成：22个trna， 2个rnas， 13个PCGs和一个假定的控制区（D-loop）。在13个PCGs中，观察到4种终止密码子（TAA、TAG、AGA、T——），除COX1以GTG起始外，其余基因均以ATG起始密码子起始。BI树表现出一致的拓扑结构，表明与芦笋和芦笋关系密切。本研究为L. fulvus的有丝分裂基因组提供了有价值的基因组信息，增强了我们对Lutjanidae科有丝分裂基因组关系的认识，并为进一步探索Lutjanus属有丝分裂基因组提供了基础参考。此外，这些数据为黄颡鱼的分类、遗传多样性研究和保护管理提供了新的分子证据和理论基础。

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来源期刊

Zoologischer Anzeiger 生物-动物学

CiteScore

2.80

自引率

7.10%

发文量

审稿时长

>12 weeks

期刊介绍： Zoologischer Anzeiger - A Journal of Comparative Zoology is devoted to comparative zoology with a special emphasis on morphology, systematics, biogeography, and evolutionary biology targeting all metazoans, both modern and extinct. We also consider taxonomic submissions addressing a broader systematic and/or evolutionary context. The overall aim of the journal is to contribute to our understanding of the organismic world from an evolutionary perspective. The journal Zoologischer Anzeiger invites suggestions for special issues. Interested parties may contact one of the editors.