Development and functional stability of a next-generation nutraceutical combining Pleurotus djamor mushroom and Limosilactobacillus fermentum strains

IF 4.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2026-01-01 Epub Date: 2025-11-30 DOI:10.1016/j.fbp.2025.11.026

Kamylla Mylena Souza Barbosa , Evandro Leite de Souza , Francinalva Dantas de Medeiros , Giuliane Moura Andrade , Jaielison Yandro Pereira da Silva , José Luiz de Brito Alves , Jhonatan Rafael Zárate-Salazar , Marcos dos Santos Lima , Maria Elieidy Gomes de Oliveira , Natália Sufiatti Holanda Cavalcanti , Maiara da Costa Lima , Yasmim Fonseca Simplicio da Silva , Fillipe de Oliveira Pereira

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Abstract

This study reports the development and evaluation of the viability and functional stability of potentially symbiotic nutraceuticals formulated with the edible mushroom Pleurotus djamor and probiotic Limosilactobacillus fermentum (139, 263, and 296). The nutraceutical (NPD) was produced by combining P. djamor powder with L. fermentum strains and freeze-drying, and its physicochemical properties, probiotic viability, and stability were assessed under storage and simulated gastrointestinal digestion. NPD presented high protein and fiber content, low fat, and phenolic compounds (epigallocatechin gallate, catechin, and epicatechin) associated with antioxidant activity. After 90 days, the viable cell counts decreased significantly at both 4 ± 0.5 °C (NPD4) and 25 ± 0.5 °C (NPD25) (p ≤ 0.05). However, flow cytometry revealed that NPD4 better preserved viable cells and phenolic stability compared to NPD25 (p ≤ 0.05). A substantial fraction of cells remained metabolically active but non-culturable under both storage conditions, with higher values consistently observed in NPD4. Although P. djamor powder provided partial protection, simulated gastrointestinal digestion still caused a progressive decline in cell counts, probiotic viability and antioxidant activity. This is the first study to combine P. djamor with a multi-strain L. fermentum. The nutraceutical strategy described herein represents a promising approach for developing next-generation nutraceuticals targeting gut health.

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新一代杏鲍菇与发酵limmosilactobacillus fermentum复合营养品的研制及功能稳定性研究

本研究报告了由食用菌侧耳菇（Pleurotus djamor）和益生菌发酵芽孢杆菌（Limosilactobacillus fermentum）配制的潜在共生营养品的开发和可行性和功能稳定性的评估（139,263和296）。采用酵母粉与发酵乳杆菌（L. fermentum）混合冷冻干燥法制备该营养品，并在贮藏和模拟胃肠消化条件下对其理化性质、益生菌活力和稳定性进行了评价。NPD具有高蛋白和纤维含量、低脂肪和与抗氧化活性相关的酚类化合物（表没食子儿茶素没食子酸酯、儿茶素和表儿茶素）。90 d后，在4 ± 0.5°C （NPD4）和25 ± 0.5°C （NPD25）条件下，活细胞计数均显著下降（p ≤ 0.05）。然而，流式细胞术显示，与NPD25相比，NPD4更好地保存了活细胞和酚稳定性（p ≤ 0.05）。在两种储存条件下，相当一部分细胞仍保持代谢活性，但不可培养，在NPD4中观察到的值始终较高。虽然大葱粉提供了部分保护，但模拟胃肠道消化仍会导致细胞计数、益生菌活力和抗氧化活性的逐渐下降。这是第一次将P. djamor与多菌株发酵乳杆菌结合的研究。本文描述的营养保健品策略代表了开发下一代针对肠道健康的营养保健品的有希望的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.