The Comparative Validity of the Sublabial Fossa to the Sublingual Triangle in Sidestream Dark Field Videomicroscopy for Measuring Microcirculatory Parameters

IF 2.4 4区医学 Q3 HEMATOLOGY

Microcirculation Pub Date : 2025-12-04 DOI:10.1111/micc.70038

Trent Payne, Ryan Homes, Raushan Lala, Ruth E. Hubbard, Mark Midwinter

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引用次数: 0

Abstract

Objective

This study compared microcirculatory measures using sidestream dark field (SDF) videomicroscopy at sublingual and sublabial regions, examining whether novice examiners can achieve comparable reliability to experienced operators and the effect of operator experience on sublabial image quality.

Methods

Microcirculatory imaging was performed using SDF videomicroscopy at the sublingual and sublabial regions. Sublingually, the probe was placed under the tongue on the mucosa of the floor of the mouth, in a stable, flat position to minimize tissue distortion. Sub-labially, the probe was positioned on the mucosa medial to the superior labial frenulum and stabilized between the central and lateral incisors. Participants were positioned at a 40-degree incline. Both novice and experienced operators captured images, and manually analyzed clips in AVA 3.2 software. All images were evaluated using the microcirculatory image quality score. Total vessel density, perfused vessel density, portion of perfused vessels, microvascular flow index, and heterogeneity index were calculated.

Results

No statistically significant differences in image quality scores were found between observers at the sublabial site. Vessel density measures demonstrated reliable inter-observer agreement, while some flow indices showed proportional bias (MFI, p = 0.01). No significant differences were detected between sublingual and sublabial regions for most measures, except for the microvascular flow index.

Conclusions

The sublabial site is a valid alternative to the sublingual region for measuring certain microcirculatory parameters, though it requires further evaluation in flow metrics.

查看原文本刊更多论文

侧流暗场视频显微镜测量微循环参数的唇下窝与舌下三角的比较有效性。

目的：本研究比较了侧流暗场（SDF）视频显微镜在舌下和下唇区域的微循环测量，考察新手检查人员是否能达到与经验丰富的操作人员相当的可靠性，以及操作人员经验对下唇图像质量的影响。方法：采用SDF视频显微镜对舌下和下唇区进行微循环成像。舌下将探针置于舌下，置于口腔底部粘膜上，处于稳定、平坦的位置，以尽量减少组织变形。唇下探针位于唇上系带内侧的粘膜上，稳定在中切牙和侧切牙之间。参与者被安排在一个40度的倾斜位置。新手和经验丰富的操作员都可以捕获图像，并在AVA 3.2软件中手动分析片段。使用微循环图像质量评分对所有图像进行评估。计算血管总密度、灌注血管密度、灌注血管比例、微血管流动指数、非均质性指数。结果：观察者在下颚部位的图像质量评分无统计学差异。血管密度测量显示出可靠的观察者间一致性，而一些流量指数显示出比例偏差（MFI, p = 0.01）。除了微血管流动指数外，舌下和下唇区域在大多数测量中没有显著差异。结论：唇下部位是测量某些微循环参数的有效替代方法，尽管它需要进一步的血流指标评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.