PhcK mediates transport of a β-5-type lignin-derived dimer in Sphingobium lignivorans SYK-6

IF 3.9 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2026-03-01 Epub Date: 2025-11-22 DOI:10.1016/j.enzmictec.2025.110784

Mitsuru Kawazoe , Masaya Fujita , Shojiro Hishiyama , Naofumi Kamimura , Eiji Masai

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Abstract

Lignin is a major component of plant cell walls; however, its heterogeneous and complex structure has hindered its efficient utilization. Recently, strategies that combine chemical pretreatment with microbial conversion to produce valuable chemicals have attracted attention. To develop an ideal microbial platform for this purpose, it is essential to elucidate the complete bacterial catabolic system for lignin-derived aromatic compounds. Here, we identified an inner membrane transporter gene involved in the uptake of a metabolite of dehydrodiconiferyl alcohol (DCA), a lignin-derived β-5 dimer, in Sphingobium lignivorans SYK-6. SLG_12820 (phcK) was found to encode a major facilitator superfamily transporter belonging to the endosomal spinster family and is regulated by PhcR, a transcriptional regulator of DCA catabolism genes. Through mutant analysis and a specific uptake assay based on PhcR effector recognition, we demonstrated that PhcK functions as an inner membrane transporter that specifically imports the DCA metabolite, 3-(2-(4-hydroxy-3-methoxyphenyl)-3-(hydroxymethyl)-7-methoxy-2,3-dihydrobenzofuran-5-yl)acrylic acid.

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PhcK介导木质素鞘SYK-6中β-5型木质素衍生二聚体的转运

木质素是植物细胞壁的主要成分；然而，其异质复杂的结构阻碍了其高效利用。近年来，将化学预处理与微生物转化相结合以生产有价值化学品的策略引起了人们的关注。为了开发一个理想的微生物平台，有必要阐明木质素衍生芳香族化合物的完整细菌分解代谢系统。在这里，我们发现了一个参与木质素衍生的β-5二聚体脱氢二硝基醇（DCA）代谢物摄取的内膜转运基因。SLG_12820 （phcK）编码一个属于内体spinster家族的主要促进体超家族转运蛋白，并受DCA分解代谢基因的转录调节因子PhcR调控。通过突变体分析和基于PhcR效应识别的特异性摄取实验，我们证明PhcK作为一种内膜转运体，特异性地进口DCA代谢物3-（2-（4-羟基-3-甲氧基苯基）-3-（羟甲基）-7-甲氧基-2,3-二氢苯并呋喃-5-基）丙烯酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.