The protective effects of α-lipoic acid against D-galactose-induced cellular senescence in human SH-SY5Y neuroblastoma cell line.

IF 2.3 Q3 CHEMISTRY, MEDICINAL

Research in Pharmaceutical Sciences Pub Date : 2025-10-20 eCollection Date: 2025-10-01 DOI:10.4103/RPS.RPS_215_24

Hanieh Nadi Yazdi, Farshad Mirzavi, Mahla Kazemian Kakhki, Amir R Afshari, Seyed Hadi Mousavi, Mohammad Soukhtanloo

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Abstract

Background and purpose: Aging is a dynamic and progressive loss of physiological integrity that leads to irreversible changes in cells and tissues, thereby increasing the risk of disability, disease, and death. Previous studies have provided evidence that D-galactose (D-gal) mimics the natural aging process in humans. On the other hand, it has been shown that α-lipoic acid (α-LA) acts as an anti-inflammatory and antioxidant compound. Therefore, this study aimed to investigate the protective effects of α-LA on D-gal-induced cellular senescence in SH-SY5Y neuroblastoma cells.

Experimental approach: Senescence was induced in SH-SY5Y cells by D-gal, and the protective effects of α-LA against D-gal toxicity were evaluated by the assays of β-galactosidase, reactive oxygen species (ROS), and antioxidant parameters in SH-SY5Y cells. In addition, the mRNA expression of Bax, Bcl-2, and p53 genes was evaluated using qRT-PCR.

Findings/results: The results revealed that α-LA at the concentrations of 62.5 and 125 μM reduced the cytotoxicity and senescence caused by D-gal. α-LA also effectively reduced the ROS generation compared to the D-gal group. Treatment with α-LA significantly modulated the levels of malondialdehyde, total thiol, and superoxide dismutase activity, which were altered by D-gal. In addition, treatment with α-LA decreased the expression of Bax and p53 genes, while increasing the expression of the Bcl-2 gene.

Conclusion and implications: Overall, the results showed that α-LA could moderate the toxic effects of D-gal by increasing the antioxidant capacity and modulating the genes involved in apoptosis, and it deserves further studies.

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α-硫辛酸对d -半乳糖诱导的人SH-SY5Y神经母细胞瘤细胞衰老的保护作用。

背景和目的：衰老是生理完整性的动态和渐进丧失，导致细胞和组织发生不可逆转的变化，从而增加残疾、疾病和死亡的风险。以前的研究已经提供了证据，证明d -半乳糖（D-gal）模仿人类的自然衰老过程。另一方面，α-硫辛酸（α-LA）具有抗炎、抗氧化的作用。因此，本研究旨在探讨α-LA对d -gal诱导的SH-SY5Y神经母细胞瘤细胞衰老的保护作用。实验方法：采用d -半乳糖诱导SH-SY5Y细胞衰老，通过对SH-SY5Y细胞β-半乳糖苷酶、活性氧（ROS）和抗氧化指标的测定，评价α-LA对d -半乳糖毒性的保护作用。此外，采用qRT-PCR检测Bax、Bcl-2和p53基因的mRNA表达。结果：α-LA在62.5 μM和125 μM浓度下均能降低D-gal引起的细胞毒性和衰老。与D-gal组相比，α-LA也有效地减少了ROS的产生。α-LA处理显著调节丙二醛、总硫醇和超氧化物歧化酶活性水平，而D-gal则改变了这些水平。此外，α-LA治疗降低了Bax和p53基因的表达，而增加了Bcl-2基因的表达。结论与意义：综上所述，α-LA可能通过增加抗氧化能力和调节凋亡相关基因来调节D-gal的毒性作用，值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in Pharmaceutical Sciences CHEMISTRY, MEDICINAL-

CiteScore

3.60

自引率

19.00%

发文量

审稿时长

34 weeks

期刊介绍： Research in Pharmaceutical Sciences (RPS) is included in Thomson Reuters ESCI Web of Science (searchable at WoS master journal list), indexed with PubMed and PubMed Central and abstracted in the Elsevier Bibliographic Databases. Databases include Scopus, EMBASE, EMCare, EMBiology and Elsevier BIOBASE. It is also indexed in several specialized databases including Scientific Information Database (SID), Google Scholar, Iran Medex, Magiran, Index Copernicus (IC) and Islamic World Science Citation Center (ISC).