Cytotoxic effects of silver nanoparticles and non-essential metals in murine macrophages

IF 4.3 4区医学 Q1 MEDICINE, LEGAL

Regulatory Toxicology and Pharmacology Pub Date : 2026-01-01 Epub Date: 2025-11-10 DOI:10.1016/j.yrtph.2025.105987

Andressa Glinski , Jessica Zablocki da Luz , Aliciane de Almeida Roque , Tugstênio Lima de Souza , Arandi Ginane Bezerra Junior , Carolina Camargo de Oliveira , Ciro Alberto de Oliveira Ribeiro , Francisco Filipak Neto

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引用次数: 0

Abstract

Silver nanoparticles (AgNPs) are widely used in nanotechnology products. However, the health risks associated with co-exposure to these emerging contaminants and environmental pollutants, such as non-essential metals, are poorly understood. The present study aimed to investigate the cytotoxicity and toxicological interaction of AgNPs (0.36 and 3.6 μg mL⁻¹) + lead (Pb²⁺, 25 and 250 μM) and AgNPs + mercury (Hg²⁺, 15 and 150 μM) using the macrophage cell line RAW 264.7 as a model. Effects were observed after a few hours (4 h) on NO levels, phagocytic activity, and DNA damage. Cell viability (24 h-exposure) was affected mainly by the higher concentrations of the contaminants and their mixtures, preceded by increases in NO levels and DNA damage, but without effects on ROS levels. Co-exposure potentiated some effects (ROS and NO levels and DNA damage), indicating toxicological interaction. These important findings must be further investigated, since the interaction of Pb²⁺ and Hg²⁺ with AgNPs from nanoproducts may impair the function of macrophages and represent a health risk for humans.

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银纳米颗粒和非必需金属对小鼠巨噬细胞的细胞毒性作用。

银纳米粒子（AgNPs）在纳米技术产品中应用广泛。然而，与这些新出现的污染物和环境污染物（如非必需金属）共同暴露有关的健康风险却知之甚少。本研究以巨噬细胞RAW 264.7为模型，研究AgNPs(0.36和3.6 μ mL-1) +铅（Pb2+， 25和250 μM）和AgNPs +汞（Hg2+， 15和150 μM）对巨噬细胞的细胞毒性和毒理学相互作用。几小时（4小时）后观察NO水平、吞噬活性和DNA损伤。细胞活力（暴露24小时）主要受到较高浓度污染物及其混合物的影响，在此之前会增加NO水平和DNA损伤，但对ROS水平没有影响。共同暴露增强了一些效应（ROS和NO水平和DNA损伤），表明毒理学相互作用。这些重要的发现必须进一步研究，因为Pb2+和Hg2+与来自纳米产品的AgNPs的相互作用可能损害巨噬细胞的功能，并对人类构成健康风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Toxicology and Pharmacology 医学-毒理学

CiteScore

6.70

自引率

8.80%

发文量

147

审稿时长

58 days

期刊介绍： Regulatory Toxicology and Pharmacology publishes peer reviewed articles that involve the generation, evaluation, and interpretation of experimental animal and human data that are of direct importance and relevance for regulatory authorities with respect to toxicological and pharmacological regulations in society. All peer-reviewed articles that are published should be devoted to improve the protection of human health and environment. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of toxicological and pharmacological compounds on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of human and environmental health. Types of peer-reviewed articles published: -Original research articles of relevance for regulatory aspects covering aspects including, but not limited to: 1.Factors influencing human sensitivity 2.Exposure science related to risk assessment 3.Alternative toxicological test methods 4.Frameworks for evaluation and integration of data in regulatory evaluations 5.Harmonization across regulatory agencies 6.Read-across methods and evaluations -Contemporary Reviews on policy related Research issues -Letters to the Editor -Guest Editorials (by Invitation)