{"title":"Increased hsa_circ_0075829 Facilitates the Progression of Gastric Cancer and Its Relationship with Helicobacter Pylori Infection.","authors":"Zhentao Li, Haihan Zhang, Zhuangmei Wang","doi":"10.1615/JEnvironPatholToxicolOncol.2025054934","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Circular RNAs (circRNAs) play a pivotal part in the advancement of multiple tumors. Nonetheless, the influence of Helicobacter pylori (H. pylori) infection on the expression of circRNA in gastric cancer remains less studied.</p><p><strong>Aim: </strong>The objective of this research is to clarify the function and underlying mechanism of circ_0075829 in the context of gastric cancer and its relation to H. pylori infection.</p><p><strong>Methods: </strong>The circ_0075829 expression in tissue specimens and cells was evaluated utilizing quantitative real-time PCR (RT-qPCR). H. pylori infection and transfection were treated in gastric cancer cells to measure circ_0075829 expression changes and their effects on cellular behaviors. Kaplan-Meier curve was conducted to assess the clinical prognostic performance of circ_0085729 in patients with gastric cancer. Bioinformatics and dua-luciferase reporter assay were performed to validate the downstream miRNA and mRNA. CCK-8 proliferation assay and transwell experiments were carried out to investigate the influence of circ_0075829-miR-149-5p-AGO1 on cellular activities.</p><p><strong>Results: </strong>Circ_0075829 expression was increased in gastric cancer and correlated with several clinical characteristics and shorter overall survival. Besides, H. pylori infection increased circ_0075829 expression in cancer cells. miR-149-5p was a direct target miRNA of circ_0075829 and was downregulated in gastric cancer. Downregulation of circ_0075829 could repress the proliferative abilities, migratory capacities, and invasion potential of cancer cells by mediating miR-149-5p to regulate AGO1 expression.</p><p><strong>Conclusion: </strong>Circ_0075829 seems to play an oncogenic role in H. pylori-associated gastric malignancies and may serve as a promising indicator for predicting outcomes in gastric cancer, which underpins a more theoretical basis for the progression of new therapeutic approaches to treating gastric cancer.</p>","PeriodicalId":94332,"journal":{"name":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","volume":"44 4","pages":"1-13"},"PeriodicalIF":2.9000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1615/JEnvironPatholToxicolOncol.2025054934","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Background: Circular RNAs (circRNAs) play a pivotal part in the advancement of multiple tumors. Nonetheless, the influence of Helicobacter pylori (H. pylori) infection on the expression of circRNA in gastric cancer remains less studied.
Aim: The objective of this research is to clarify the function and underlying mechanism of circ_0075829 in the context of gastric cancer and its relation to H. pylori infection.
Methods: The circ_0075829 expression in tissue specimens and cells was evaluated utilizing quantitative real-time PCR (RT-qPCR). H. pylori infection and transfection were treated in gastric cancer cells to measure circ_0075829 expression changes and their effects on cellular behaviors. Kaplan-Meier curve was conducted to assess the clinical prognostic performance of circ_0085729 in patients with gastric cancer. Bioinformatics and dua-luciferase reporter assay were performed to validate the downstream miRNA and mRNA. CCK-8 proliferation assay and transwell experiments were carried out to investigate the influence of circ_0075829-miR-149-5p-AGO1 on cellular activities.
Results: Circ_0075829 expression was increased in gastric cancer and correlated with several clinical characteristics and shorter overall survival. Besides, H. pylori infection increased circ_0075829 expression in cancer cells. miR-149-5p was a direct target miRNA of circ_0075829 and was downregulated in gastric cancer. Downregulation of circ_0075829 could repress the proliferative abilities, migratory capacities, and invasion potential of cancer cells by mediating miR-149-5p to regulate AGO1 expression.
Conclusion: Circ_0075829 seems to play an oncogenic role in H. pylori-associated gastric malignancies and may serve as a promising indicator for predicting outcomes in gastric cancer, which underpins a more theoretical basis for the progression of new therapeutic approaches to treating gastric cancer.
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