Fading on the beach: pigmentation plasticity adjusts coloration to substrate type in coastal Western spadefoot toads.

Abstract

Animal species exhibit a wide range of coloration and patterns, which are under strong selection and often linked to individual quality, influencing mate choice, recognition, signaling, and interspecific interactions such as predator avoidance. In amphibians, coloration can vary based on individual traits as well as environmental conditions, including the coloration of their environment. In this study, we described the dorsal coloration (body coloration reticulated by dark patches) in 676 Western spadefoot toads (Pelobates cultripes) from the French Atlantic coast, comparing color variations across different substrates. In addition, we assessed color change in 18 toads transferred between bright and dark substrates. We demonstrated that the dorsal coloration varies based on capture locations (beach vs. inland) and associated substrate types (bright vs. dark), suggesting background color matching. We showed more pronounced effects in females, which might reflect varying predation risks. Experimentally, we showed that individuals can rapidly adjust their coloration to match the substrate within 24 h. Rapid color changes in response to substrate type indicate significant pigmentation plasticity. Bright individuals from sandy substrates showed less dorsal background (body) color change than dark inland individuals, while patch coloration responded differently depending on the substrate of origin. These findings highlight the complex interactions between substrate type, sex, and pigmentation plasticity. These interactions have potential costs and benefits, which might be linked to melanin production, which warrant further investigation.