Repair of DNA single-strand and double-strand breaks in the Chinese hamster xrs 5 mutant cell line as determined by DNA unwinding

Nina D. Costa, Peter E. Bryant
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引用次数: 32

Abstract

The DNA unwinding technique has been used to measure the induction and repair of DNA strand breaks by X-rays in the X-ray sensitive (xrs 5) mutant and its parent CHO K1 line of Chinese hamster cells. Results show that frequency of induction of DNA strand breaks was the same for both cell lines. The repair of single-strand breaks was found to be slightly slower in xrs 5 over the first 20 min after X-ray exposure, but the level of repair of ssb reached after an incubation of 1h following X-ray exposure in xrs 5 was the same as in CHO K1. Our results also show that the rate of repair of DNA double-strand breaks in xrs 5 cells was clearly slower than that in CHO K1, supporting the conclusion of Kemp et al. (1984) who used the neutral elution technique, that xrs 5 is defective in the repair pathway of DNA double-strand breaks.

中国仓鼠xrs5突变细胞系DNA单链和双链断裂的DNA解绕修复
利用DNA解绕技术测定了中国仓鼠x射线敏感突变体(xrs5)及其亲本CHO K1细胞系在x射线诱导和修复DNA链断裂的过程。结果表明,两种细胞系DNA链断裂的诱导频率相同。单链断裂的修复在x射线暴露后的头20分钟内被发现在xrs 5中稍慢,但在x射线暴露后的孵育1h后,xrs 5中ssb的修复水平与CHO K1相同。我们的研究结果还表明,xrs 5细胞的DNA双链断裂修复速率明显慢于CHO K1,这支持了Kemp等人(1984)使用中性洗脱技术的结论,即xrs 5在DNA双链断裂修复途径中存在缺陷。
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