Optimized Genome Assembly Protocol for Cryptosporidium.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2026-01-01 DOI:10.1007/978-1-0716-4824-7_9

Wanyi Huang, Lihua Xiao

引用次数: 0

Abstract

Whole-genome sequence data have made significant contributions to studies of the biology and transmission of Cryptosporidium spp. Well-assembled genomes, free of sequence ambiguity and structural errors, are critical for comparative genomic analysis at the species and subtype levels. The optimized method for Cryptosporidium genome assembly described here comprises several individual protocols to generate chromosome- and contig-level genomes.

查看原文本刊更多论文

隐孢子虫基因组组装方案优化。

全基因组序列数据为隐孢子虫的生物学和传播研究做出了重要贡献。完整组装的基因组，没有序列模糊和结构错误，对物种和亚型水平的比较基因组分析至关重要。本文描述的隐孢子虫基因组组装的优化方法包括几种单独的方案，以产生染色体和contig水平的基因组。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.