A Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Scheme for Epidemiological Investigations of Cryptosporidium parvum.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2026-01-01 DOI:10.1007/978-1-0716-4824-7_8

Harriet Risby, Guy Robinson, Rachel M Chalmers

引用次数: 0

Abstract

A multi-locus variable-number tandem-repeat analysis (MLVA) scheme has been validated and used for subtyping Cryptosporidium parvum, to infer linkage between cases, and suspected sources, in outbreaks and for epidemiological surveillance. Here, we describe the multiplex polymerase chain reaction (PCR) assays, fragment sizing, and bioinformatics to identify alleles and create a seven-locus MLVA profile for each specimen. The PCRs to produce amplicons for Sanger sequencing to confirm new alleles are also described.

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小隐孢子虫流行病学调查的多位点可变数串联重复分析（MLVA）方案。

一种多位点可变数串联重复分析（MLVA）方案已得到验证，并用于小隐孢子虫亚型分型，以推断病例和疑似来源之间的联系，并用于疫情和流行病学监测。在这里，我们描述了多重聚合酶链反应（PCR）测定，片段大小和生物信息学来识别等位基因，并为每个标本创建一个七个位点的MLVA谱。还描述了产生扩增子的pcr，用于Sanger测序以确认新的等位基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.