Hsuan Fu, Chun-Yin Yang, Pamela Berilyn So, Chia-Hung Yen, Tzu-Hui Wu, Feng-Lin Yen
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引用次数: 0
Abstract
Ethnopharmacological relevance: Clitoria ternatea L. (Fabaceae), commonly known as butterfly pea, has a well-established role in Ayurvedic and Southeast Asian traditional medicine. While its internal uses-for enhancing memory and reducing inflammation-are well documented, its external applications for treating skin irritations, wounds, and inflammation are less scientifically substantiated, despite historical use in India, Thailand, and Indonesia.
Aim of the study: This study aimed to evaluate the dermal safety and anti-pollution efficacy of Clitoria ternatea flower (CTF) extracts prepared via traditional water digestion (CTFW) and ultrasound-assisted ethanol extraction (CTFE).
Materials and methods: In vitro safety assessments were conducted using OECD-validated methods, including Direct Peptide Reactivity Assay (DPRA), ARE-Nrf2 luciferase assay, MTT cytotoxicity, and phototoxicity assays. Dermal absorption was analyzed using a Franz diffusion cell, followed by calculation of systemic exposure dose (SED) and margin of safety (MoS). Anti-pollution efficacy was evaluated via intracellular ROS inhibition and western blot analysis of particulate matter (PM)-induced oxidative stress and inflammatory markers in HaCaT keratinocytes.
Results: Both extracts were non-sensitizing and showed favorable dermal safety without systemic exposure. Although CTFE exhibited phototoxicity at high concentrations, CTFW demonstrated superior ROS-scavenging activity and significantly inhibited PM-induced inflammatory and aging-related proteins. MoS estimation, based on in vitro percutaneous absorption data, further suggested a favorable safety margin for both extracts in topical application.
Conclusion: These findings support the ethnopharmacological use of Clitoria ternatea for skin-related conditions and highlight its promise as a bioactive cosmetic ingredient. CTFW, in particular, offers enhanced anti-pollution efficacy and a stronger safety profile.
期刊介绍：
The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.
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