Protein production in transgenic chickens mediated through primordial germ cells and embryonic stem cells

IF 1.8 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Protein expression and purification Pub Date : 2026-02-01 Epub Date: 2025-10-20 DOI:10.1016/j.pep.2025.106828

Mengran Fang , Xinyue Chen , Yuan Liu, Hui Yuan

引用次数: 0

Abstract

The inherent advantages of transgenic chickens, combined with the increasing demand for therapeutic proteins, are driving significant progress in transgenic chicken bioreactors. Primordial germ cells (PGCs) and embryonic stem cells (ESCs) are two pivotal carrier cells in the production of transgenic chicken bioreactors. Transgenic chickens have successfully produced therapeutic proteins using genetically modified and transferred PGCs. Meanwhile, considerable progress has been made in expressing therapeutic proteins using exogenous gene integration mediated by ESCs. Both carrier cells facilitate the efficient process of transgenic chickens. Here, we reviewed the characteristics, current applications, comparisons, and future directions of these two carrier cells, providing theoretical foundations and practical guidance for advancing development in the fields of chicken bioreactors.

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通过原始生殖细胞和胚胎干细胞介导转基因鸡的蛋白质生产。

转基因鸡的固有优势，加上对治疗性蛋白需求的不断增长，推动了转基因鸡生物反应器的重大进展。原始生殖细胞（PGCs）和胚胎干细胞（ESCs）是转基因鸡生物反应器生产中的两种关键载体细胞。转基因鸡已经成功地利用转基因和转移的PGCs生产出治疗性蛋白质。与此同时，利用ESCs介导的外源基因整合表达治疗性蛋白也取得了相当大的进展。这两种载体细胞促进了转基因鸡的高效过程。本文综述了这两种载体细胞的特点、应用现状、比较及未来发展方向，为推进鸡生物反应器领域的发展提供理论基础和实践指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Protein expression and purification 生物-生化研究方法

CiteScore

3.70

自引率

6.20%

发文量

120

审稿时长

32 days

期刊介绍： Protein Expression and Purification is an international journal providing a forum for the dissemination of new information on protein expression, extraction, purification, characterization, and/or applications using conventional biochemical and/or modern molecular biological approaches and methods, which are of broad interest to the field. The journal does not typically publish repetitive examples of protein expression and purification involving standard, well-established, methods. However, exceptions might include studies on important and/or difficult to express and/or purify proteins and/or studies that include extensive protein characterization, which provide new, previously unpublished information.