Molecular characterization and antibiogram profiling of hypervirulent Klebsiella pneumoniae isolated from clinical-environmental interface

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics Pub Date : 2025-10-10 DOI:10.1016/j.egg.2025.100413

Prisweta Bhattacharjee , Rajkumari Elizabeth , Sayani Roy , Anupam Das Talukdar , Manabendra Dutta Choudhury , Amitabha Bhattacharjee

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Abstract

This study investigates the prevalence, virulence gene profile, and antibiotic resistance of hypervirulent Klebsiella pneumoniae (hvKp) strains isolated from drainage water near healthcare facilities in Southern Assam. A total of 32 K. pneumoniae isolates were identified, with 19 carrying virulence genes out of which 13 isolates carrying multiple virulence genes, including those responsible for iron acquisition and siderophore biosynthesis. Antibiogram profiling revealed high resistance rates, particularly to norfloxacin (78.94 %), followed by ampicillin (73.68 %) and ciprofloxacin (68.42 %), while imipenem and meropenem retained higher effectiveness, despite some isolates showing resistance. These findings highlight the clinical-environmental reservoirs of multidrug-resistant, hypervirulent K. pneumoniae strains, posing significant challenges for infection control and treatment in healthcare settings.

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临床-环境界面分离的高致病性肺炎克雷伯菌的分子特征和抗生素谱分析

本研究调查了从阿萨姆邦南部医疗设施附近的排水中分离出的高毒力肺炎克雷伯菌（hvKp）菌株的流行率、毒力基因谱和抗生素耐药性。共鉴定出32株肺炎克雷伯菌，其中19株携带毒力基因，其中13株携带多毒力基因，包括负责铁获取和铁载体生物合成的毒力基因。抗生素谱分析显示高耐药率，特别是对诺氟沙星（78.94%），其次是氨苄西林（73.68%）和环丙沙星（68.42%），而亚胺培南和美罗培南保持较高的有效性，尽管一些分离株表现出耐药性。这些发现突出了多药耐药、高毒力肺炎克雷伯菌菌株的临床环境储存库，对卫生保健机构的感染控制和治疗提出了重大挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecological Genetics and Genomics Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： Ecological Genetics and Genomics publishes ecological studies of broad interest that provide significant insight into ecological interactions or/ and species diversification. New data in these areas are published as research papers, or methods and resource reports that provide novel information on technologies or tools that will be of interest to a broad readership. Complete data sets are shared where appropriate. The journal also provides Reviews, and Perspectives articles, which present commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context. Topics include: -metagenomics -population genetics/genomics -evolutionary ecology -conservation and molecular adaptation -speciation genetics -environmental and marine genomics -ecological simulation -genomic divergence of organisms