Old and new tactics of CRISPR-centric competition between bacteria and bacteriophages

IF 7.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-12-01 Epub Date: 2025-10-15 DOI:10.1016/j.sbi.2025.103168

Iktae Kim , Jeong-Yong Suh

引用次数: 0

Abstract

The CRISPR-Cas system provides adaptive immunity for prokaryotes against mobile genetic elements (MGEs) such as bacteriophages and plasmids. As a countermeasure, MGEs have evolved various anti-CRISPR (Acr) mechanisms that neutralize the CRISPR-mediated immunity. Canonical Acr proteins block target binding of Cas proteins in a stoichiometric or enzymatic manner. New findings reveal that Acr also disintegrates functional Cas complexes, induces promiscuous target binding, and mimics Cas proteins and crRNA with defective mutations. Here, we summarize a broad repertoire of structural and functional mechanisms underlying CRISPR-centric competition, highlighting recent discoveries of molecular machinery that modulates CRISPR function.

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细菌和噬菌体之间以crispr为中心的竞争的新旧策略。

CRISPR-Cas系统为原核生物提供了针对移动遗传元件（MGEs）（如噬菌体和质粒）的适应性免疫。作为对策，MGEs进化出各种抗crispr （Acr）机制来中和crispr介导的免疫。典型Acr蛋白以化学计量学或酶促的方式阻断Cas蛋白的靶结合。新的研究结果表明，Acr还能分解功能性Cas复合物，诱导混杂靶标结合，并模拟Cas蛋白和crRNA的缺陷突变。在这里，我们总结了以CRISPR为中心的竞争背后的广泛的结构和功能机制，重点介绍了最近发现的调节CRISPR功能的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation