Plant-Derived Nanovesicle Enhanced Microribonucleic Acid (MicroRNA) Transfer from Nasal Cavity to the Brain.

Abstract

The nasal-to-brain route for drug delivery is potentially useful for the treatment of brain disorders. Not only chemically modified molecules but also nanosized carrier vesicles, such as cell culture-derived exosomes, as small molecules, could be delivered via this route. The focus of the present study was to evaluate the potential of vesicular plant-derived nanoparticles (PDNPs), which have been identified as multivesicular bodies, for transporting molecules via the nasal-to-brain route. PDNPs were isolated from the edible parts of onion (Allium cepa), cherry tomato (Lycopersicon esculentum var. cerasiforme), Delaware grape (Vitis labrusca "Delaware"), and grapefruit (Citrus × paradisi) using commercial kits without ultracentrifugation. Recombinant exogenous microRNA (cel-miR-39) was encapsulated into PDNPs by electroporation. Cel-miR-39-incorporated PDNPs were administered to adult male C57BL/6J mice via intranasal instillation or intravenous injection, and then tissue samples were collected. Cel-miR-39 transportation efficiency was evaluated by quantitative RT-PCR. Intranasal instillation was found to be more effective than intravenous injection for microRNA delivery to the brain. The onion-derived nanoparticle was the most effective transporter of microRNA to the olfactory bulb and caudal brain. The transportation potential and kinetics of other molecules (therapeutic drugs) using onion-derived nanoparticles are of future interest.