Neuromodulatory efficacy of Bacopa monniera extract against streptozotocin-induced neuronal dysfunction in SH-SY5Y cells: Implications for diabetic neuropathy

IF 2.7 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2026-03-01 Epub Date: 2025-10-13 DOI:10.1016/j.tiv.2025.106166

H.S. Bhumika , M.D. Pandareesh

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引用次数: 0

Abstract

Diabetic neuropathy (DN), a major complication of diabetes mellitus, is characterized by progressive neuronal damage driven by hyperglycemia-induced oxidative stress, mitochondrial dysfunction, and advanced glycation end product (AGE) accumulation. Bacopa monniera (Scrophulariaceae), enriched with the neuroactive saponin Bacoside A (BA), has demonstrated neuroprotective potential. This study explored the molecular mechanisms underlying the neuroprotective effects of B. monniera extract (BME) against streptozotocin (STZ)-induced toxicity in SH-SY5Y neuroblastoma cells. In silico ADMET analysis revealed that BA and its sapogenins possess favorable pharmacokinetic profiles, including enhanced absorption, reduced toxicity, and improved clearance, supporting their bioactive role in BME. Pretreatment with BME (25 μg/mL) significantly (p < 0.01) reduced STZ-induced mitochondrial (51.24 %) and membrane (41.69 %) damage, as shown by MTT and LDH assays. BME markedly reduced intracellular ROS, protein carbonylation, and lipid peroxidation (p < 0.001), while restoring mitochondrial membrane potential, ATP levels, enzymatic antioxidant levels, and glutathione content. Furthermore, BME upregulated brain-derived neurotrophic factor (BDNF) expression and inhibited AGE formation. Collectively, these findings highlight the antioxidant, antiglycation, and neurotrophic actions of BME, underscoring its promise as a multi-targeted phytotherapeutic candidate for DN management.

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假马齿苋提取物对链脲佐菌素诱导的SH-SY5Y细胞神经功能障碍的神经调节作用：对糖尿病神经病变的影响。

糖尿病神经病变（DN）是糖尿病的主要并发症，其特征是由高血糖诱导的氧化应激、线粒体功能障碍和晚期糖基化终产物（AGE）积累驱动的进行性神经元损伤。马齿苋（Bacopa monniera）富含神经活性皂苷马齿苋苷A (Bacoside A， BA)，具有神经保护作用。本研究探讨了monniera提取物（BME）抗STZ诱导的SH-SY5Y神经母细胞瘤细胞毒性的分子机制。计算机ADMET分析显示，BA及其皂苷元具有良好的药代动力学特征，包括增强吸收、降低毒性和提高清除率，支持其在BME中的生物活性作用。BME预处理（25 μg/mL）显著(p

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.