The complete mitochondrial genome of Plagiorchiidae gen. (Digenea, Plagiorchiidae) cercariae larvae in snails and its phylogenetic implications.

IF 1.2 4区医学 Q4 PARASITOLOGY

Systematic Parasitology Pub Date : 2025-10-15 DOI:10.1007/s11230-025-10253-9

Janelle Laura J Gacad, Natalia I Yurlova, Maria Camille M Calilung, Mudjekeewis D Santos, Misako Urabe

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Abstract

We determined the complete mitochondrial (mt) genome of a species of Plagiorchiidae, obtained from Lymnaea stagnalis snail collected at Lake Chany, western Siberia. The cercariae were morphologically close to Opisthioglyphe ranae (Frölich, 1791), but the species identification is still pending. The mitogenome is 13,849 base pairs in length. This genome includes 12 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and one non-coding region. Notably, 20 tRNA genes produce transcribed products with conventional cloverleaf structures, while two tRNA genes have unpaired D-arms. The gene atp8 is absent, and the remaining genes are transcribed in the same direction. The A + T content of the Plagiorchiidae gen. mt genome is 65.64%. The complete mtDNA of Plagiorchiidae gen. is shorter than that of all other related xiphidiate species compared in this study. Phylogenetic analysis based on the whole mitogenome revealed that Plagiorchiidae gen. clusters closely with species from the genera Plagiorchis and Glypthelmins. Additionally, the analysis of the 28S rRNA gene indicated that it belongs to Plagiorchiidae and is particularly close to Dolichosaccus (syn. Lecithopyge). This study reports the complete mitochondrial genome of Plagiorchiidae gen., providing a valuable resource of molecular markers for future research on the taxonomy, population genetics, and systematics of the family Plagiorchiidae.

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钉螺斜纹尾蚴幼虫线粒体全基因组及其系统发育意义。

我们测定了一种斜纹蜗牛科的全线粒体基因组，该物种来自西伯利亚西部的Chany湖（Lake Chany）的lynaea alis蜗牛。尾蚴在形态上接近于棘目（Opisthioglyphe ranae）（Frölich, 1791），但种类鉴定尚待确定。有丝分裂基因组的长度为13,849个碱基对。该基因组包括12个蛋白质编码基因（PCGs）、22个转移RNA （tRNA）基因、2个核糖体RNA （rRNA）基因和1个非编码区。值得注意的是，20个tRNA基因产生具有传统三叶草结构的转录产物，而两个tRNA基因具有未配对的d臂。基因atp8缺失，其余基因转录方向相同。抄袭科（Plagiorchiidae gen. mt）基因组A + T含量为65.64%。本研究所比较的剑蝗科所有近缘种中，剑蝗科的完整mtDNA较短。基于全有丝分裂基因组的系统发育分析表明，抄花科与抄花属和Glypthelmins属的种关系密切。此外，28S rRNA基因分析表明，它属于抄袭科，与Dolichosaccus （syn. Lecithopyge）特别接近。本研究报道了抄花科线粒体全基因组，为今后抄花科的分类、群体遗传学和系统学研究提供了有价值的分子标记资源。

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来源期刊

Systematic Parasitology 医学-寄生虫学

CiteScore

2.20

自引率

23.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Systematic Parasitology publishes papers on the systematics, taxonomy and nomenclature of the following groups: Nematoda (including plant-parasitic), Monogenea, Digenea, Cestoda, Acanthocephala, Aspidogastrea, Cestodaria, Arthropoda (parasitic copepods, hymenopterans, mites, ticks, etc.), Protozoa (parasitic groups), and parasitic genera in other groups, such as Mollusca, Turbelleria, etc. Systematic Parasitology publishes fully illustrated research papers, brief communications, and fully illustrated major revisions. In order to maintain high standards, all contributors describing new taxa are asked to state clearly where the holotype is deposited and to make paratypes available for examination by the referees. It is recognized that, in some cases, this may cause problems for the authors, but it is hoped that by adhering to this rule authors may be protected against rapid synonymy of their taxa, and the types will be preserved for posterity.