Protective and therapeutic effects of betanin nanoparticles in an alzheimer's rat model: modulation of behavior and expression of AQP4, BDNF, SIRT6, and Seladin-1.

IF 3.5 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Metabolic brain disease Pub Date : 2025-10-11 DOI:10.1007/s11011-025-01715-6

Nasim Abedimanesh, Seyed Alireza Miri, Ali Mohammadi, Fatemeh Shahmohammadi, Hossein Danafar, Mohammad Reza Eskandari, Sajjad Hejazi, Sina Andalib, Behrooz Motlagh

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引用次数: 0

Abstract

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive decline, oxidative stress, and neuroinflammation. Betanin, a natural antioxidant, has shown neuroprotective potential, but its clinical use is limited by poor bioavailability. This study investigates the effects of betanin-loaded nanomicelles, designed to enhance brain delivery, in a scopolamine-induced rat model of AD. Nanomicelles were synthesized and characterized using TEM, DLS, and FT-IR. Rats received either pre- or post-treatment with betanin nanomicelles, free betanin, donepezil, or saline. Cognitive performance was assessed using the Morris Water Maze. Gene expression levels of AQP4, BDNF, SIRT6, and Seladin-1 were measured using real-time PCR, and antioxidant activity was evaluated by assessing glutathione (GSH) and glutathione reductase (GR) in hippocampal tissue. Betanin nanomicelles improved spatial memory, increased BDNF and SIRT6 expression, and reduced AQP4 levels, indicating potential neuroprotection. Seladin-1 expression was notably elevated in the pre-treatment group, suggesting support for neuronal survival. Antioxidant assays showed restoration of GSH and GR activity. These findings suggest that betanin nanomicelles may enhance cognitive function and modulate neuroprotective pathways more effectively than free betanin, supporting their potential as a novel therapeutic strategy for AD.

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甜菜素纳米颗粒在老年痴呆症大鼠模型中的保护和治疗作用：AQP4、BDNF、SIRT6和Seladin-1的行为和表达的调节

阿尔茨海默病（AD）是一种以认知能力下降、氧化应激和神经炎症为特征的神经退行性疾病。甜菜素是一种天然抗氧化剂，具有神经保护作用，但其临床应用受到生物利用度差的限制。本研究在东莨菪碱诱导的AD大鼠模型中，研究了装载甜菜素的纳米胶束的作用，该纳米胶束旨在增强脑递送。合成了纳米胶束，并用TEM、DLS和FT-IR对其进行了表征。大鼠分别接受甜菜素纳米胶束、游离甜菜素、多奈哌齐或生理盐水治疗前后。使用Morris水迷宫评估认知表现。实时荧光定量PCR检测AQP4、BDNF、SIRT6、Seladin-1基因表达水平，通过测定海马组织谷胱甘肽（GSH）和谷胱甘肽还原酶（GR）检测抗氧化活性。甜菜素纳米胶束改善空间记忆，增加BDNF和SIRT6表达，降低AQP4水平，提示潜在的神经保护作用。治疗前组Seladin-1表达明显升高，提示支持神经元存活。抗氧化试验显示GSH和GR活性恢复。这些发现表明，甜菜素纳米胶束可能比游离甜菜素更有效地增强认知功能和调节神经保护通路，支持其作为AD新治疗策略的潜力。

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来源期刊

Metabolic brain disease 医学-内分泌学与代谢

CiteScore

5.90

自引率

5.60%

发文量

248

审稿时长

6-12 weeks

期刊介绍： Metabolic Brain Disease serves as a forum for the publication of outstanding basic and clinical papers on all metabolic brain disease, including both human and animal studies. The journal publishes papers on the fundamental pathogenesis of these disorders and on related experimental and clinical techniques and methodologies. Metabolic Brain Disease is directed to physicians, neuroscientists, internists, psychiatrists, neurologists, pathologists, and others involved in the research and treatment of a broad range of metabolic brain disorders.