Design of Peptide Inhibitors Using Expression Tags: Structure of the Complex of Phosphopantetheine Adenylyltransferase with 17-Residue Expression-Tag Peptide and Citric Acid at 2.10 Å Resolution.

Abstract

Phosphopantetheine adenylyltransferase (PPAT) catalyzes the transfer of an adenylyl group from adenosine triphosphate (ATP) to 4'-phosphopantetheine (PNS) to generate dephosphocoenzyme A (dPCoA) and pyrophosphate (PP_i). The dPCoA is required for the biosynthesis of coenzyme A (CoA), which is a vital cofactor in several essential biochemical reactions. PPAT enzyme from Enterobacter spp. (EbPPAT), cloned with a 30-residue-long N-terminal tag, was purified and crystallized. The structure determination of EbPPAT revealed the presence of six protein molecules, A, B, C, D, E, and F, in the asymmetric unit, which formed three homodimers designated as A-B, C-D and E-F. At the N-termini of molecules B and F, 17 additional residues belonging to the expression tag were observed. These 17-residue segments of molecules B and F were located deep inside the PNS-binding sites of the adjacent molecules. In addition to this, six citric acid (CIT) molecules were observed in the ATP-binding sites of all six EbPPAT molecules. Thus, the 17-mer peptide and CIT molecules filled the substrate-binding cleft of EbPPAT completely. In order to estimate the binding affinity, the 17-mer tag peptide was synthesized. The K_D value for the 17-mer peptide was found to be 1.7 × 10^-8 M. The K_D value for the CIT molecule was 2.13 × 10^--5 M. These values indicated higher binding affinities of the 17-mer peptide and CIT molecule than those of the substrates, PNS and ATP, respectively. These results suggest that expression-tag fragments can be used to design the required peptide inhibitors of enzymes.