White and brown adipose tissue share a convergent fibro-adipogenic progenitor population.

IF 6.2 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

EMBO Reports Pub Date : 2025-10-08 DOI:10.1038/s44319-025-00591-6

Hoang V Bui, Julia K Hansen, Valentina Lo Sardo, Andrea Galmozzi

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引用次数: 0

Abstract

Adipose tissue heterogeneity has emerged as a central factor in regulating adipose tissue function in physiology and pathophysiology, yet tools to model and study this diversity in vitro remain limited. Here, we performed single-cell RNA sequencing on cultured primary white and brown preadipocytes to assess how in vitro conditions impact progenitor identity. We identified two major subpopulations in both depots: committed adipogenic precursors (CAPs) and fibro-adipogenic progenitor-like cells (FAPLs). Remarkably, FAPLs were also present in brown adipose tissue, expanding the known landscape of progenitor populations in this depot. Trajectory and regulon analyses revealed that both white and brown FAPLs exhibit similar pro-fibrotic, stress-responsive signatures and diverge early from proliferating progenitor states. Integration of datasets showed that FAPLs from both depots cluster together, emphasizing their conserved identity, while CAPs remain depot-specific. Comparison to previously published in vivo single-cell datasets revealed that these in vitro populations, including brown adipose FAPLs, correspond to adipose-resident progenitor subtypes, validating the physiological relevance of this model for studying adipose tissue heterogeneity and development.

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白色和棕色脂肪组织共享一个趋同的纤维脂肪祖细胞群。

脂肪组织异质性已成为调节脂肪组织生理和病理生理功能的核心因素，但体外模型和研究这种多样性的工具仍然有限。在这里，我们对培养的原代白色和棕色前脂肪细胞进行了单细胞RNA测序，以评估体外条件如何影响祖细胞身份。我们在两个储存库中确定了两个主要的亚群：固定脂肪生成前体（cap）和纤维脂肪生成祖细胞样细胞（FAPLs）。值得注意的是，FAPLs也存在于棕色脂肪组织中，扩大了该储存库中祖先种群的已知景观。轨迹和调控分析显示，白色和棕色fapl都表现出相似的促纤维化、应激反应特征，并且与增殖祖细胞状态早期分化。数据集的整合表明，来自两个仓库的fapl聚集在一起，强调了它们的保守性，而cap仍然是特定于仓库的。与先前发表的体内单细胞数据集的比较显示，这些体外群体，包括棕色脂肪FAPLs，对应于脂肪居住的祖细胞亚型，验证了该模型在研究脂肪组织异质性和发育方面的生理相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

EMBO Reports 生物-生化与分子生物学

CiteScore

11.20

自引率

1.30%

发文量

267

审稿时长

1 months

期刊介绍： EMBO Reports is a scientific journal that specializes in publishing research articles in the fields of molecular biology, cell biology, and developmental biology. The journal is known for its commitment to publishing high-quality, impactful research that provides novel physiological and functional insights. These insights are expected to be supported by robust evidence, with independent lines of inquiry validating the findings. The journal's scope includes both long and short-format papers, catering to different types of research contributions. It values studies that: Communicate major findings: Articles that report significant discoveries or advancements in the understanding of biological processes at the molecular, cellular, and developmental levels. Confirm important findings: Research that validates or supports existing knowledge in the field, reinforcing the reliability of previous studies. Refute prominent claims: Studies that challenge or disprove widely accepted ideas or hypotheses in the biosciences, contributing to the correction and evolution of scientific understanding. Present null data: Papers that report negative results or findings that do not support a particular hypothesis, which are crucial for the scientific process as they help to refine or redirect research efforts. EMBO Reports is dedicated to maintaining high standards of scientific rigor and integrity, ensuring that the research it publishes contributes meaningfully to the advancement of knowledge in the life sciences. By covering a broad spectrum of topics and encouraging the publication of both positive and negative results, the journal plays a vital role in promoting a comprehensive and balanced view of scientific inquiry.