A Thermosensitive Gel Containing Biodegradable Nanoparticles Carrying Calcium Hydroxide as Antibacterial Intracanal Therapy.

IF 7.1 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International endodontic journal Pub Date : 2025-10-07 DOI:10.1111/iej.70032

Xavier Roig-Soriano, Luis María Delgado, Firas Elmsmari, Fernando Duran-Sindreu, Miriam Teulé, Marta Espina, Gerard Esteruelas, Maria Luisa García, José Antonio González Sánchez, Elena Sánchez-López

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引用次数: 0

Abstract

Aim: To characterise and evaluate mucoadhesive strength and antibacterial properties of a nanotechnological formulation for endodontic disinfection based on biodegradable nanoparticles dispersed in a thermosensitive gel containing calcium hydroxide (Ca(OH)₂-NPs-gel).

Methodology: Morphology of Ca(OH)₂-NPs-gel was studied using transmission electron microscopy. Moreover, Ca(OH)₂-NPs-gel was sterilised using gamma irradiation (25 kGy), and the stability after the sterilisation process was studied by measuring Ca(OH)₂-NPs-gel average size, polydispersity index, zeta potential and encapsulation efficiency. To assess the ex vivo mucoadhesive strength, extracted single-rooted human teeth were used to measure the force necessary to separate the formulation from the teeth. In addition, the short-time stability of Ca(OH)₂-NPs-gel was evaluated monthly, analysing entrapment efficacy, backscattering and transmittance of Ca(OH)₂-NPs-gel stored at different temperatures (4°C, 25°C and 37°C). Furthermore, the antibacterial analysis of Ca(OH)₂-NPs-gel was performed against Enterococcus faecalis inoculated in extracted human single-root teeth and evaluated by confocal and scanning electron microscopy. Finally, the metabolic activity of bacteria was studied through a resazurin assay to evaluate bacterial survival after treatment.

Results: Ca(OH)₂-NPs-gel owned a round shape and a smooth surface without particle aggregation. Sterilisation did not induce an alteration in Ca(OH)₂-NPs-gel physicochemical properties and Ca(OH)₂-NPs-gel presented a high adhesion strength. In addition, 4°C was the best temperature to store Ca(OH)₂-NPs-gel. Regarding the antibacterial therapeutic efficacy, Ca(OH)₂-NPs-gel possesses suitable antibacterial properties, indicating that it efficiently reduces bacterial biofilms.

Conclusion: Calcium hydroxide-loaded PLGA nanoparticles dispersed in a thermosensitive gel have been developed, optimised and characterised, obtaining excellent antibacterial properties and achieving bacterial disinfection levels similar to those of commercial formulations.

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含有可生物降解纳米颗粒的热敏凝胶携带氢氧化钙作为抗菌肛管治疗。

目的：表征和评价一种基于分散在含氢氧化钙的热敏凝胶（Ca(OH)2- nps凝胶）中的可生物降解纳米颗粒的根管消毒纳米配方的粘着强度和抗菌性能。方法：用透射电镜研究了Ca(OH)2- nps凝胶的形貌。采用γ辐照（25 kGy）对Ca(OH)2- nps -凝胶进行灭菌，通过测定Ca(OH)2- nps -凝胶的平均粒径、多分散性指数、zeta电位和包封效率，研究灭菌后的稳定性。为了评估离体黏附强度，使用拔出的单根人牙来测量将配方与牙齿分离所需的力。此外，每月评估Ca(OH)2- nps -凝胶的短期稳定性，分析Ca(OH)2- nps -凝胶在不同温度（4°C、25°C和37°C）下的捕获效率、后向散射和透射率。此外，还对Ca(OH)2- nps -凝胶对提取的人单根牙接种的粪肠球菌进行了抗菌分析，并通过共聚焦和扫描电镜进行了评价。最后，通过reazurin试验研究细菌的代谢活性，以评估治疗后细菌的存活率。结果：Ca(OH)2- nps -凝胶呈圆形，表面光滑，无颗粒聚集。灭菌未引起Ca(OH)2- nps -凝胶理化性质的改变，Ca(OH)2- nps -凝胶具有较高的粘附强度。4℃是Ca(OH)2- nps凝胶的最佳保存温度。在抗菌治疗效果方面，Ca(OH)2- nps -凝胶具有合适的抗菌性能，表明其有效地减少了细菌生物膜。结论：开发、优化和表征了分散在热敏凝胶中的氢氧化钙负载PLGA纳米颗粒，获得了优异的抗菌性能，达到了与商业配方相似的细菌消毒水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International endodontic journal 医学-牙科与口腔外科

CiteScore

10.20

自引率

28.00%

发文量

195

审稿时长

4-8 weeks

期刊介绍： The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.