Distinct waves of ovarian follicles contribute to mouse oocyte production.

IF 6.4 1区 生物学 Q1 BIOLOGY
eLife Pub Date : 2025-10-07 DOI:10.7554/eLife.107352
Qi Yin, Allan C Spradling
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Abstract

The earliest growing mouse follicles, wave 1, rapidly develop in the ovarian medulla, while the great majority, wave 2, are stored for later use as resting primordial follicles in the cortex. Wave 1 follicles are known to mostly undergo atresia, a fate sometimes associated with the persistence of steroidogenic theca cells, but this connection is poorly understood. We characterized wave 1 follicle biology using tissue clearing, lineage tracing, and scRNA-seq to clarify their contributions to offspring and steroidogenesis. Wave 1 follicles, lineage-marked by E16.5 Foxl2 expression in granulosa cells, reach preantral stages containing theca cell layers by 2 weeks. Atresia begins about a week later, during which 80-100% of wave 1 follicles degrade their oocytes, turn over most granulosa cells, but retain theca cells which expand in number together with interstitial gland cells in the medulla. During puberty (5 weeks), these cells ultrastructurally resemble steroidogenic cells and highly express androgen biosynthetic genes. Unexpectedly, the Foxl2 lineage tag also marked about 400 primordial follicles, located near the medullary-cortical boundary, that become the earliest activated wave 2 follicles. These 'boundary' or 'wave 1.5' follicles generate 70-100% of the earliest mature oocytes, while fewer than 26 wave 1 follicles with oocytes survive. Consistent with their largely distinct fates in steroid or oocyte production, granulosa cells of antral wave 1 and 2 follicles differentially express multiple genes, including Wnt4 and Igfbp5.

不同的卵泡波有助于小鼠卵母细胞的产生。
最早生长的小鼠卵泡(波1)在卵巢髓质中迅速发育,而绝大多数(波2)作为休眠的原始卵泡储存在皮层中供以后使用。已知波1卵泡大多数经历闭锁,这种命运有时与甾体源性卵泡细胞的持续存在有关,但这种联系尚不清楚。我们使用组织清除、谱系追踪和scRNA-seq来表征波1卵泡生物学,以阐明它们对后代和类固醇形成的贡献。波1卵泡,在颗粒细胞中以E16.5 Foxl2表达为谱系标记,在2周时达到含有卵泡细胞层的前腔期。闭锁约一周后开始,在此期间80-100%的波1卵泡降解卵母细胞,翻转大部分颗粒细胞,但保留数量增加的卵泡细胞和髓质间质腺细胞。在青春期(5周),这些细胞在超微结构上类似于类固醇细胞,并高度表达雄激素生物合成基因。出乎意料的是,Foxl2谱系标签还标记了大约400个位于髓质-皮层边界附近的原始卵泡,这些卵泡成为最早激活的波2卵泡。这些“边界”或“波1.5”卵泡产生70-100%最早成熟的卵母细胞,而含有卵母细胞的波1卵泡存活的不到26个。与它们在类固醇或卵母细胞产生中截然不同的命运相一致,中波1和2卵泡颗粒细胞差异表达多种基因,包括Wnt4和Igfbp5。
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来源期刊
eLife
eLife BIOLOGY-
CiteScore
12.90
自引率
3.90%
发文量
3122
审稿时长
17 weeks
期刊介绍: eLife is a distinguished, not-for-profit, peer-reviewed open access scientific journal that specializes in the fields of biomedical and life sciences. eLife is known for its selective publication process, which includes a variety of article types such as: Research Articles: Detailed reports of original research findings. Short Reports: Concise presentations of significant findings that do not warrant a full-length research article. Tools and Resources: Descriptions of new tools, technologies, or resources that facilitate scientific research. Research Advances: Brief reports on significant scientific advancements that have immediate implications for the field. Scientific Correspondence: Short communications that comment on or provide additional information related to published articles. Review Articles: Comprehensive overviews of a specific topic or field within the life sciences.
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