A unified rodent atlas reveals the cellular complexity and evolutionary divergence of the dorsal vagal complex.

IF 6.4 1区 生物学 Q1 BIOLOGY
eLife Pub Date : 2025-10-07 DOI:10.7554/eLife.106217
Cecilia Hes, Abigail J Tomlinson, Lieke Michielsen, Hunter J Murdoch, Fatemeh Soltani, Maia V Kokoeva, Paul V Sabatini
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引用次数: 0

Abstract

The dorsal vagal complex (DVC) is a region in the brainstem comprised of an intricate network of specialized cells responsible for sensing and propagating many appetite-related cues. Understanding the dynamics controlling appetite requires deeply exploring the cell types and transitory states harbored in this brain site. We generated a multi-species DVC cell atlas using single-nuclei RNA-sequencing, by curating and harmonizing mouse and rat data, which includes >180,000 cells and 123 cell identities at 5 granularities of cellular resolution. We report unique DVC features such as Kcnj3 expression in Ca+-permeable astrocytes as well as new cell populations like neurons co-expressing Th and Cck, and a leptin receptor-expressing neuron population in the rat area postrema which is marked by expression of the progenitor marker, Pdgfra. In summary, our findings demonstrate a high degree of complexity within the DVC and provide a valuable tool for the study of this metabolic center.

统一的啮齿动物图谱揭示了背迷走神经复合体的细胞复杂性和进化分化。
背迷走神经复合体(DVC)是脑干中的一个区域,由复杂的特化细胞网络组成,负责感知和传播许多与食欲有关的信号。了解控制食欲的动力学需要深入探索大脑这个部位的细胞类型和短暂状态。我们使用单核rna测序,通过整理和协调小鼠和大鼠的数据,生成了一个多物种DVC细胞图谱,其中包括bb180,000个细胞和123个细胞身份,细胞分辨率为5个颗粒。我们报道了独特的DVC特征,如Kcnj3在Ca+渗透性星形细胞中的表达,以及新的细胞群,如共表达Th和Cck的神经元,以及在大鼠脑后区域表达瘦素受体的神经元群,其标志是祖细胞标记物Pdgfra的表达。总之,我们的研究结果表明了DVC的高度复杂性,并为研究该代谢中心提供了有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
eLife
eLife BIOLOGY-
CiteScore
12.90
自引率
3.90%
发文量
3122
审稿时长
17 weeks
期刊介绍: eLife is a distinguished, not-for-profit, peer-reviewed open access scientific journal that specializes in the fields of biomedical and life sciences. eLife is known for its selective publication process, which includes a variety of article types such as: Research Articles: Detailed reports of original research findings. Short Reports: Concise presentations of significant findings that do not warrant a full-length research article. Tools and Resources: Descriptions of new tools, technologies, or resources that facilitate scientific research. Research Advances: Brief reports on significant scientific advancements that have immediate implications for the field. Scientific Correspondence: Short communications that comment on or provide additional information related to published articles. Review Articles: Comprehensive overviews of a specific topic or field within the life sciences.
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