Microbial Community Profiles of Biofilms from Hot Springs: 16S and 18S rRNA Amplicon Sequencing Data.

IF 1.4 Q3 MULTIDISCIPLINARY SCIENCES

Data in Brief Pub Date : 2025-09-19 eCollection Date: 2025-10-01 DOI:10.1016/j.dib.2025.112093

Nur Azmira Alea Nurhazli, Jia Hao Tan, Mohd Farizal Kamaroddin, Mohd Shahir Shamsir, Amira Suriaty Yaakop, Kian Mau Goh

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引用次数: 0

Abstract

This article presents microbial diversity data from biofilms collected from the sides or outflows of several Malaysian hot springs, with temperatures ranging from 38 to 56 °C and pH values between 7.1 and 8.7. Genomic DNA was extracted from the biofilms and subjected to 16S V3-V4 and 18S V4 amplicon sequencing using the Illumina NovaSeq 6000 platform. Reads were processed with various bioinformatic tools including QIIME2, and eventually, amplicon sequence variants (ASVs) were identified. In almost all analyzed biofilms, approximately 50% of the total ASVs belonged to Cyanobacteriota and Chloroflexota, except for one biofilm, labeled DTO, which was dominated by Pseudomonadata and Cyanobacteriota. Besides bacteria, the data also suggest the presence of various eukaryotic organisms, including small animals such as nematodes, rotifers, and arthropods; fungi and fungus-like organisms such as Ascomycota, Zoopagomycota, Oomycota, and Cryptomycota; as well as photosynthetic eukaryotes from the Viridiplantae group. This dataset serves as a valuable resource for microbial ecology studies in hot spring biofilms and is openly available for reuse, providing a foundation for future research on microbial diversity and functional roles in geothermal ecosystems.

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温泉生物膜微生物群落特征：16S和18S rRNA扩增子测序数据

本文介绍了从马来西亚几个温泉的侧面或流出处收集的生物膜的微生物多样性数据，这些温泉的温度范围为38至56°C， pH值为7.1至8.7。从生物膜中提取基因组DNA，使用Illumina NovaSeq 6000平台进行16S V3-V4和18S V4扩增子测序。利用QIIME2等多种生物信息学工具对Reads进行处理，最终鉴定出扩增子序列变异（amplicon sequence variant, asv）。在几乎所有分析的生物膜中，大约50%的asv属于蓝藻门和氯氟藻门，除了一个标记为DTO的生物膜，该生物膜以假单胞菌和蓝藻门为主。除了细菌，数据还表明存在各种真核生物，包括小动物，如线虫、轮虫和节肢动物；真菌和类真菌生物，如子囊菌门、动物菌门、卵菌门和隐菌门；以及来自绿植物组的光合真核生物。该数据集可作为温泉生物膜微生物生态学研究的宝贵资源，并可公开利用，为未来地热生态系统微生物多样性和功能作用的研究提供基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Data in Brief MULTIDISCIPLINARY SCIENCES-

CiteScore

3.10

自引率

0.00%

发文量

996

审稿时长

70 days

期刊介绍： Data in Brief provides a way for researchers to easily share and reuse each other''s datasets by publishing data articles that: -Thoroughly describe your data, facilitating reproducibility. -Make your data, which is often buried in supplementary material, easier to find. -Increase traffic towards associated research articles and data, leading to more citations. -Open up doors for new collaborations. Because you never know what data will be useful to someone else, Data in Brief welcomes submissions that describe data from all research areas.