Cu-BDC MOF Immobilized Cytochrome-c: A Promising Platform for Sub-Nanomolar Level Electrochemical Sensing of NADH

IF 2.8 4区化学 Q3 CHEMISTRY, PHYSICAL

Electrocatalysis Pub Date : 2025-08-13 DOI:10.1007/s12678-025-00968-0

Shaima Hameed, Adil Amin Wani, Aamir Y. Bhat, Pravin P. Ingole, Mohsin Ahmad Bhat

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引用次数: 0

Abstract

Electrochemical sensing is a promising approach for the selective and highly sensitive sensing of oxidized and reduced states of Nicotinamide adenine dinucleotide (NAD⁺/NADH). However, the limited selectivity, large overpotential requirements and the electrode fouling concerns associated with the till date reported NADH-specific electrodes continue to impede their potential utility for the design and development of fast, inexpensive and highly reliable point of care devices for electrochemical sensing of NAD⁺ and NADH. Herein we present a simple covalent functionalization approach for the design and development of Cytochrome-c (Cyt-c) functionalized Cu-BDC MOF (Cyt-c/Cu-BDC) as a novel Cu-Fe based bio-mimic for electrochemical sensing of NADH. Our detailed physical, chemical and electrochemical investigations carried out over the so designed Cyt-c/Cu-BDC composite establish it as an electronically conducting, electrochemically stable redox-active electrode material with an exceptional activity towards the selective and ultrasensitive electrochemical sensing of NADH. We demonstrate the practical utility of Cyt-c/Cu-BDC composite for accurate and sensitive electrochemical sensing of NADH in the pico-molar concentration range. The herein demonstrated extremely low LOD (10.4 pM), high sensitivity (12.02 ± 0.119 μA nM⁻¹ cm⁻²), good anti-interference ability and prolonged stability of the Cyt-c/Cu-BDC composite is far superior than the till date reported electrochemical sensors for NADH. These features qualify Cyt-c/Cu-BDC composite as a promising electrode material for the design of point-of-care NADH sensing devices for clinical diagnostics.

Graphical Abstract

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Cu-BDC MOF固定化细胞色素c：亚纳摩尔水平NADH电化学传感的一个有前景的平台

电化学传感是一种具有选择性和高灵敏度的检测烟酰胺腺嘌呤二核苷酸（NAD+/NADH）氧化和还原态的方法。然而，迄今为止报道的NADH特异性电极的有限选择性、大过电位要求和电极污染问题继续阻碍了它们在设计和开发快速、廉价和高度可靠的护理点设备方面的潜在应用，这些设备用于NAD+和NADH的电化学传感。本文提出了一种简单的共价功能化方法，用于设计和开发细胞色素c （Cyt-c）功能化Cu-BDC MOF (Cyt-c/Cu-BDC)，作为一种新型的Cu-Fe基生物模拟物，用于NADH的电化学传感。我们对设计的Cyt-c/Cu-BDC复合材料进行了详细的物理、化学和电化学研究，证明它是一种导电、电化学稳定的氧化还原活性电极材料，对NADH的选择性和超灵敏的电化学传感具有特殊的活性。我们证明了Cyt-c/Cu-BDC复合材料在微摩尔浓度范围内精确、灵敏地电化学检测NADH的实用性。结果表明，该复合材料具有极低的LOD （10.4 pM）、高灵敏度（12.02±0.119 μA nM - 1 cm - 2）、良好的抗干扰能力和较长的稳定性，远远优于目前报道的NADH电化学传感器。这些特点使Cyt-c/Cu-BDC复合材料成为设计用于临床诊断的即时护理NADH传感装置的有前途的电极材料。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Electrocatalysis CHEMISTRY, PHYSICAL-ELECTROCHEMISTRY

CiteScore

4.80

自引率

6.50%

发文量

审稿时长

>12 weeks

期刊介绍： Electrocatalysis is cross-disciplinary in nature, and attracts the interest of chemists, physicists, biochemists, surface and materials scientists, and engineers. Electrocatalysis provides the unique international forum solely dedicated to the exchange of novel ideas in electrocatalysis for academic, government, and industrial researchers. Quick publication of new results, concepts, and inventions made involving Electrocatalysis stimulates scientific discoveries and breakthroughs, promotes the scientific and engineering concepts that are critical to the development of novel electrochemical technologies. Electrocatalysis publishes original submissions in the form of letters, research papers, review articles, book reviews, and educational papers. Letters are preliminary reports that communicate new and important findings. Regular research papers are complete reports of new results, and their analysis and discussion. Review articles critically and constructively examine development in areas of electrocatalysis that are of broad interest and importance. Educational papers discuss important concepts whose understanding is vital to advances in theoretical and experimental aspects of electrochemical reactions.