Electrochemical Determination of Uric Acid in Biological Samples by Using Chitosan-Nickel(II) Complex Modified MWCNTs Paste Electrode

IF 2.8 4区化学 Q3 CHEMISTRY, PHYSICAL

Electrocatalysis Pub Date : 2025-08-26 DOI:10.1007/s12678-025-00978-y

Shumaila Noureen, Abdul Niaz, Iqbal Zaman, Muhammad Tariq

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Abstract

A highly sensitive MWCNTs paste electrode (MWCNTPE) modified with chitosan-nickel complex (Chit-Ni²⁺) was designed for the efficient electrochemical determination of uric acid (UA). The MWCNTPE surface is drop-coated with the Chit-Ni²⁺ complex. The electroanalytical performance of Chit-Ni²⁺ complex modified MWCNTPE toward UA is thoroughly analyzed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Experimental results reveal that Chit-Ni²⁺ complex modified MWCNTPE exhibits superior electrochemical performance toward determination of UA as compared to bare MWCNTPE. The voltammetric sensitivity of Chit-Ni²⁺ complex modified MWCNTPE toward UA oxidation was significantly improved, with a typical peak potential of 0.39 V (vs. SCE) in LiCl solution (0.1 M) at pH 2.4. Chit-Ni²⁺ complex modified MWCNTPE exhibited a linear current response (R² ~ 0.999) in the range of 0.05–144.3 µM UA. The limit of detection (LOD) limit for the Chit-Ni²⁺ complex modified MWCNTPE is found to be 0.01 µM. The Chit-Ni²⁺ complex modified MWCNTPE was highly selective for the electrochemical determination of UA even in the presence of other potential biomolecular interfering species.

Graphical Abstract

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壳聚糖-镍（II）配合物修饰MWCNTs膏体电极电化学测定生物样品中的尿酸

设计了一种壳聚糖-镍配合物修饰的高灵敏度MWCNTPE电极（Chit-Ni2+），用于高效电化学测定尿酸。MWCNTPE表面滴涂有Chit-Ni2+络合物。采用循环伏安法（CV）和差分脉冲伏安法（DPV）深入分析了Chit-Ni2+配合物修饰的MWCNTPE对UA的电分析性能。实验结果表明，Chit-Ni2+配合物修饰的MWCNTPE在测定UA方面具有优于裸MWCNTPE的电化学性能。Chit-Ni2+配合物修饰的MWCNTPE对UA氧化的伏安敏感性显著提高，在pH 2.4的LiCl溶液（0.1 M）中，其典型峰电位为0.39 V （vs. SCE）。Chit-Ni2+配合物修饰的MWCNTPE在0.05 ~ 144.3µM UA范围内具有良好的线性电流响应（R2 ~ 0.999）。Chit-Ni2+配合物修饰的MWCNTPE的检出限为0.01µM。Chit-Ni2+络合物修饰的MWCNTPE对UA的电化学测定具有很高的选择性，即使存在其他潜在的生物分子干扰物质。图形抽象

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来源期刊

Electrocatalysis CHEMISTRY, PHYSICAL-ELECTROCHEMISTRY

CiteScore

4.80

自引率

6.50%

发文量

审稿时长

>12 weeks

期刊介绍： Electrocatalysis is cross-disciplinary in nature, and attracts the interest of chemists, physicists, biochemists, surface and materials scientists, and engineers. Electrocatalysis provides the unique international forum solely dedicated to the exchange of novel ideas in electrocatalysis for academic, government, and industrial researchers. Quick publication of new results, concepts, and inventions made involving Electrocatalysis stimulates scientific discoveries and breakthroughs, promotes the scientific and engineering concepts that are critical to the development of novel electrochemical technologies. Electrocatalysis publishes original submissions in the form of letters, research papers, review articles, book reviews, and educational papers. Letters are preliminary reports that communicate new and important findings. Regular research papers are complete reports of new results, and their analysis and discussion. Review articles critically and constructively examine development in areas of electrocatalysis that are of broad interest and importance. Educational papers discuss important concepts whose understanding is vital to advances in theoretical and experimental aspects of electrochemical reactions.